Isolation, Biochemical Characterization and Production of Immobilised β-Amylase Chitosan Beads Using Bacteria from Waste Water Effluents for its Industrial Production Aspect

Abstract

Aims: An amylase, the enzyme that catalyses the hydrolysis of starch into sugars are produced in both animals and plants and as well as in some bacteria also. Nowadays, the use of amylase enzyme in different industrial sectors and particularly in controlling the industrial water pollution has been increased as this enzyme is effective against different types of industrial effluents such as wastes from dairies, confectionaries, municipal wastes, bakery and so on which are expelled out in water without giving any proper treatment. As, the production of synthetic amylase enzyme for this purpose is quite costly, in this study, the waste water effluent was used to isolate the amylase producing bacteria, hence, decreasing the cost. Methodology: The samples were taken from drains coming from bakery, municipal waste, etc, and five bacterias were isolated at dilution 10-6  which were named accordingly as Tan1, Tan2, Tan3, Tan4 and Tan5 further followed by Gram staining and biochemical characterization tests for further confirmation of amylase producing bacteria followed by the immobilisation of the β-amylase enzyme produced by the bacteria. Results and Conclusion: Two bacterias were identified as amylase producing, Tan1 and Tan2, in which Gram positive bacteria showed higher amylase production at 30°C(Tan1). Compared to the free β-amylase, the immobilised β-amylase enzyme showed broader pH and temperature ranges, enhanced thermal stability, better storage stability, reusability and higher accessibility of the substrate to the immobilised β-amylase. Improved activity recovery and enzymatic properties of immobilised β-amylase chitosan beads in present study holds a promising future in industrial applications.