Enhancement the Antibacterial Potential of the Biosynthesized Silver Nanoparticles Using Hydrophilic Polymers

Abstract

Silver nanoparticles are well recognized for its antibacterial activity, especially against pathogenic bacteria. In the present study, silver nanoparticles (AgNPs) were biologically synthesized by using the aqueous leaves extract of Datura stramonium followed by capping with one of the hydrophilic polymers: Polyethylene glycol (PEG) and polyvinyl pyrrolidone (PVP). The biosynthesized nanoparticles were characterized by UV–Visible Spectroscopy, X-ray diffraction (XRD) and Transmission Electron Microscope (TEM). Antibacterial activity against Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterococcus sp. and Staphylococcus aureus was determined by a microtitre well method and disc diffusion method. The results revealed that the antibacterial activity of silver nanoparticles is dependent on their concentration and were active against Gram negative and Gram positive bacteria. The antibacterial activity of coated AgNPs was increased in comparison with uncoated.