Isolation and Antibiogram of Bacteria Isolated from Processed and Unprocessed Cow-Skin (Ponmo) in Ogbese Market

Abstract

The use of ponmo as major meat source to average Nigerians if processed under unhygienic condition might result in disease outbreak. Inadequate and under-utilization of food nutrients needed by human for normal body functions has resulted in malnutrition and outbreak of diseases. Fifty samples of cowhides comprising forty processed (ponmo) and ten unprocessed were sourced from Ogbese Market, Ondo State, Nigeria. The microbial analyses were carried out using serial dilution and pour plate methods. The 0.1ml of the diluents was aseptically plated on molten nutrient agar and incubated temperature. Pure cultures were obtained by repeated streaking on the appropriate microbiological media. Antibiotics sensitivity pattern on the bacterial isolates were carried out using commercial antibiotics. The highest microbial load from unprocessed Ponmo with fur was 3.5 x105 while the processed Ponmo was 1.5 x 105cfu/ml. Staphylococcus aureus, Streptococcus mitis, Salmonella typhimorium, Bacillus subtilis, Escherichia coli, Staphylococcus epidermidis, Shigella dysenteriae and Micrococcus leteus were isolated. Ciprofloxacin showed inhibitory potency on the entire isolates with Staphylococcus aureus showing high resistance to most of the antibiotics.