Comparison of Use of Agglutination of Mammalian Erythrocytes Plus Heat Stability Test and Use of Agglutination of Mammalian Erythrocytes Plus Erythrocyte Elution Time to Characterize Isolates of Newcastle Disease Virus

Abstract

Aim: For a simpler, more rapid and more accurate method of characterizing  new isolates of Newcastle disease virus (NDV), agglutination of mammalian erythrocytes (HA)  plus heat stability  test  and  agglutination of  mammalian erythrocytes  plus  erythrocytes  elution time (EET)  were compared with  use of Intra-cerebral pathogenicity index (ICPI) to characterize the isolates.  Materials and Methods: NDV isolates characterized by their ICPI were re-characterized by HA of mammalian erythrocytes plus heat stability test and by HA of mammalian erythrocytes plus EET. Rate of agreement of each of the two combinations with ICPI was calculated. Results: HA of mammalian erythrocytes plus heat stability agreed with ICPI in characterizing 10 of the 12 NDV isolates (83.3%) while use of HA of mammalian erythrocytes plus EET agreed with ICPI in all the 12 isolates (100%). Conclusion: It was concluded  that  use of combination of  agglutination  of mammalian erythrocytes  and  EET to characterize NDV isolates  has better agreement with use of ICPI to characterize the virus  than use of combination of agglutination of mammalian erythrocytes  and  heat stability test.