Growth Rate and Antifungal Activities of Acetone Extracts of Ocimum gratissimum (Scent Leaf) and Allium sativum (Garlic) on Cassava and Banana Peels Formulated Media
Ebele Linda Okoye, Bright Obidinma Uba, Uzoma Collins Dike, Chimezie Justin Eziefule
Journal of Advances in Microbiology · pp. 19–29 · Published 28 Apr 2020
10.9734/jamb/2020/v20i430233Abstract
Aim: To study the growth rate and antifungal activities of acetone extracts of Ocimum gratissimum (scent leaf) and Allium sativum (garlic) on cassava and banana peels formulated media. Study Design: Nine treatments and control designs were set up in triplicates and incubated at 25 for 72 h. The nine treatments and control set ups designated as Cassava Glucose Agar, CGA), Banana Glucose Agar, (BGA) and Control (Potato Dextrose Agar, PDA) were used to screen for the growth rate and antifungal activities of plant extracts. Place and Duration of Study: Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Uli Nigeria between June, 2019 and August, 2019. Methodology: The research study was carried on two formulated media obtained from cassava and banana peels and PDA) using proximate analysis, pour plate technique, agar plug diffusion technique and agar well diffusion technique. Results: The results revealed that the cassava and banana peels contained nutrients that can meet the nutritional conditions for fungi cultivation. The PDA statistically (P < 0.05) had higher mean radial growth (32.33 mm) and growth rate (0.449 h-1) than BGA (14.33 mm; 0.199 h-1) followed by CGA (14.16 mm; 0.197 h-1) in most of the test fungal isolates. The scent leaf acetone extract and nystatin antifungal had higher zones of inhibition (25.00 mm) than garlic acetone extract (24.00 mm) on the tested fungal isolates. There was no statistical significance at P < 0.05 on the inhibition zones of the extracts and Nystatin on the formulated media and PDA showing that the extracts possess comparable antifungal activities to the Nystatin. Conclusion: Thus, our formulated media were comparable to PDA with regards to antifungal activity of the acetone extracts and nystatin and could be utilized as an alternative and cheap ideal reference media for mycological assays.
Cited by 1
A Sophia, Suraini, R Yogica · Journal of Physics: Conference Series · 2021
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