Pharmacokinetic and Analytical Evaluation of Cinchocaine HCl in Ointment Using Reversed-Phase HPLC

Abstract

A reversed-phase HPLC method has been developed for the separation and simultaneous determination of the local anesthetic Cinchocaine in pharmaceutical formulations. The primary objective of this study was to establish a simple, fast, efficient, and reliable HPLC analytical method for the determination of Cinchocaine in its pharmaceutical dosage form. The analysis was conducted using a reversed-phase C18 column (Luna, 250×4.6 mm, 5μm, 1000A, Phenomenex, Inc.) with a mobile phase consisting of a 50:50 (v/v) mixture of Phosphate buffer (pH 2.5) and Acetonitrile, flowing at 1.0 mL/min. Detection was performed using a UV detector set at 250 nm. The developed method demonstrated effective separation of Cinchocaine from other components in just 2.9 minutes. This method was successfully employed for the determination of Cinchocaine in ointment formulations, showcasing its potential for routine analysis in quality control settings. The rapid separation and reliable results indicate that this HPLC method can be utilized for both routine analysis and further research on local anesthetic formulations, ensuring consistent quality and efficacy in pharmaceutical products.