The Effects of Starvation- Induced Stress with G. kola Extracts and Ketogenic Diet on Some Oxidative Stress Markers in Male Wistar Rats

Abstract

Aim/Objective: This study investigated the Effects of Induced starvation with G.Kola extracts and ketogenic diet administration on oxidative stress markers. Methods: A total of 49 wistar rats acclimatized for two weeks weighing between 145-161g                   were used for this study. The KD was prepared using 1kg margarine versus 1kg normal                      feeds mixed together while the oxidative stress markers were determine by spectrophotometric approach. Results: The findings from this study shows decrease in MDA among groups administered 25mg (0.006808µmol/ml) but however increases at 50mg (0.0082 µmol/ml) and 100mg (0.008235 µmol/ml) G.kola extract doses compared with control (0.007527 µmol/ml).Also, regarding SOD there was a decrease at 25mg(1.764µ/ml) dose with no changes at 50mg whereas there was an increase at 100mg/kg(1.772µ/l) in comparison with the control group though not significant. Further observation from this study shows a high level of MDA among group fed with ketogenic diet (0.0081 µmol/ml) compared with the control (0.0075 µmol/ml) respectively. The level of MDA continue to increase among the 9hrs starved group (0.011095 µmol/ml) (control: 0.006865 µmol/ml) and the 18hrs group (0.00922 µmol/ml) above that of the control (0.009188 µmol/ml) though not significant.Moreso, there was nonsignificant increase in SOD among ketogenic fed group without starvation (1.771µ/ml) in relation to control (1.769µ/ml) and the 9hrs starvation group (1.785µ/ml) (1.767µ/ml). However we observed a decline in 18hrs starved group(1.761µ/ml) compared with control of 1.770µ/ml in SOD with no significant values. A weak positive correlation was observed in SOD versus the change in body weight among groups fed with ketogenic diet while a strong negative significant correlation between MDA and body weight was observed with r-value of -0.5917 and p value of 0.02. Conclusion: Ketogenic fed diet increases oxidative stress markers such as MDA in both non fasting and starvation induced hours. This increase was also observed among G.kola extract consumption groups of 50 and 100mg but however decreases at 25mg dose regimen daily.