Detection of FUS-1 (OXA-85), a Class D Beta-lactamase from Fusobacterium nucleatum Subspecies Polymorphum in Nigeria
Francisca O. Nwaokorie, Kofoworola O. Savage, Muinah A. Fowora, Patricia O. Ayanbadejo, Folasade T. Ogunsola, Akitoye O. Coker
Microbiology Research Journal International · pp. 492–500 · Published 25 Jul 2013
10.9734/BMRJ/2013/3599Abstract
Aims: Beta-lactamase production and subsequent resistance to β-lactam drugs has been a global concern in the treatment of Gram negative anaerobes. The aim of this study was to identify F. nucleatum strains producing Class D β-lactamase through the detection of FUS-1 (OXA-85) resistance gene. Place and Duration of Study: Department of Preventive Dentistry, Lagos University Teaching Hospital, Idi-Araba, between February 2010 and November 2010. Methodology: Twenty two oral clinical samples were obtained from patients with chronic periodontitis who admitted to previous use of amoxicillin. Antibacterial susceptibility of the bacterial isolates was determined by E-test on Brucella Blood agar. Amplification of the bacterial DNA was carried out by PCR using F. nucleatum species-specific primer, FUS-1 specific for blaFUS-1 and strain-specific primers for subspecies nucleatum,, fusiforme, polymorphum and vincentii. Results: From the 19 samples collected, F. nucleatum was isolated, and the identity of the isolates was confirmed by PCR. Four of the isolates produced similar bands with the control strain, 3 (15.7%) strains were able to produce amplication with FUS-1 primer specific for blaFUS-1 gene found in β-lactamase producing F. nucleatum subsp. polymorphum. Conclusion: This study shows the presence of class D β-lactamase producing F. nucleatum species in Nigeria.
Cited by 2
Francisca Nwaokorie, K. Okunade, U. Edet · Babcock University Medical Journal · 2023
S. Jiang, Jumei Zeng, Xuedong Zhou · Journal of dentistry research · 2018
Related research
- Genotypic Analysis of Klebsiella pneumoniae among Patients Admitted in Critical Care Settings in a Tertiary Care Center of Bangladesh — shares topic coverage
- Phenotypic Identification and Detection of BlaCTX and TetM in Klebsiella Species from Urine Samples of Patients in Ile-Ife, Nigeria — shares topic coverage
- Investigating Carriage, Contamination, Antimicrobial Resistance and Assessment of Colonization Risk Factors of Campylobacter spp. in Broilers from Selected Farms in Thika, Kenya — shares topic coverage
- Molecular Epidemiology of Fluoroquinolones Resistance Genes among Non-typhoidal Salmonella Species from Humans and Poultry Faeces in Ido-Ekiti, Ekiti State — shares topic coverage
- Molecular Characterization of Antibiotic Resistance Genes in Non-typhoidal Salmonella Isolates from Human Samples in Bangui, Central African Republic (2019–2023) — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
2
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.