Frequency of Two Genetic Polymorphisms of CYP1A2 Gene in Iranian Population

Abstract

Aim: The present study was conducted to explore the allelic and genotypic frequencies of CYP1A2 gene polymorphisms, CYP1A2*1C (−3860 G>A) and CYP1A2*1F (−163 C>A), among 200 healthy Iranian volunteers. Study Design: Completely randomized design. Place and Duration of Study: Place – Biotechnology Department, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran. Duration – June, 2011 to May, 2013. Methodology: DNA was extracted from the blood samples by salting-out method. Primers and probes were designed by the Primer Express® Software v3.0.1. Allelic discrimination of the CYP1A2 alleles was performed by Real-Time PCR, and the data were analyzed by the SPSS 16.00 software. Results: The frequency of each polymorphism in Iranian population was found to be 0.05 and 0.3 for CYP1A2*1C and CYP1A2*1F, respectively. Our data did not show any difference between the Iranian, Turkish, and Egyptian populations in CYP1A2*1C polymorphism; also, CYP1A2*1F polymorphism analysis did not show any differences between Iranian, Turkish, Egyptian, Chinese, Serbian, and Japanese populations. Allele frequency in our population was in equilibrium according to Hardy–Weinberg principle (P=0.05). Conclusion: This is the first study analyzing the CYP1A2 clinically important allelic variants in Iranian population. Our results indicate that functional significance of these alleles should be evaluated in various populations. It is necessary to determine the relationship between these variants and CYP1A2-related drug metabolism in associated groups.