Identification and Characterization of Water Borne Pathogen
Shivangi Saxena, Dipjyoti Chakraborty
South Asian Journal of Research in Microbiology · pp. 7–12 · Published 21 Dec 2023
10.9734/sajrm/2023/v17i4334Abstract
Aims: To carry out isolation and characterization of bacteria from water samples. Study Design: Collection of water samples from different water sources, isolation of bacteria and establishment of pure culture, identification of bacteria Place and Duration of Study: Sample collected from Hingotiya and Palai village of Tonk district, Rajasthan July-August, 2018. Methodology: For initial screening of bacterial population, standard methods namely serial dilution and streaking methods were used, for identification, colony morphology and molecular characterization (16s rRNA gene) was done. Results: The bacteriological analysis revealed that Enterobacter cloacae was present in the collected tap-water. Conclusion: Presence of E. cloacae in tap water necessitates recommendation of frequent sanitation, treatment, management of water sources, and routine regular bacteriological assessment of all water sources for consumption as a drinking water.
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