Efficacy of Various Concentrations of Honey Solution in the Fixation of Some Selected Tissues in Wistar Rats

Abstract

Introduction: Honey is thought to preserve the cells by preventing autolysis and putrefaction. Thus, studies have shown that honey has a good preventive putrefactive property mainly because honey contains seven tetracycline derivatives, fatty acids, lipids, amylases and ascorbic acid and hydrogen peroxide. Therefore, honey is used as an agent for preventing autolysis and putrefaction. Aim: This research aims to investigate the efficacy of some concentrations of the honey solution in fixation of some tissues (Such as kidney, liver, heart, lungs and muscle). Methodology: A Wistar rat was sacrificed by vertical abdominal incision and the above-mentioned organs ware fixed in 10% formalin (positive control), 10% honey and 20% honey this grouped the research specimen into 3 groups. Histological examinations of these organs were carried out.  Results: Almost all the histological sections fixed in honey solution revealed normal nuclear as well as a cytoplasmic outline as compared with formalin-fixed organs, except for muscle which shown some level of swollen and fragmenting tissue.