Improving the Recombinant Protein Expression of Human Galectin-3 in BL21 Bacterial Host System
Praveen Kumar Vemuri, Nikhil Reddy Varakala, Divyanshu Dhakate, Tripura Ravavarapu, Faina Philberta Dumpala, Susmita Sri Muddana, Haritha Bommepalli, Spurthi Modiboyana
Journal of Pharmaceutical Research International · pp. 111–115 · Published 11 Dec 2020
10.9734/jpri/2020/v32i3430970Abstract
Background: Regardless of the broad explore in the territory of glycobiology concerning structure and capacity of glycans, lectins and glycosylation forms, numerous viewpoints are still left unexplored. Aim: In this study, we analyzed the effect of shuttle vector on the secretion of human galectin recombinant protein. Methods: The galectin was expressed in E. coli BL21 by growing the bacterial culture in SOC medium and purified by nickel-based affinity chromatography due to its His-tag. Results: After cell lysis the protein was identified as a single 29 KDa band by 12% SDS-PAGE. Conclusion: Characterization studies clearly revealed that the purified protein was indeed galectin 3.
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Alexander A. Charbonneau, Elizabeth J. Reicks, John F. Cambria · Protein Expression and Purification · 2024
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