Isolation, Purification of Scopulariopsis fimicola and Effect of Cropping Period on Calocybe indica

Abstract

The Calocybe indica (Milky Mushroom) affected by large number of biotic and abiotic factors during cultivation. Among them, white plaster mould caused by Scopulariopsis fimicola encounter the compost and casing soil during cultivation and adversely affect the spawn run, growth and yield of C. indica. The present research work on various aspects of milky mushroom cultivation was conducted during 2019-21. June and August months (29.20- 35.80C temperature and 73.00- 85%relative humidity) are most suitable for milky mushroom cultivation. The associated mycoflora was isolated from infected casing soil, collected from farmers cultivating milky mushroom house/field. Eleven associated mycoflora viz., Aspergillus niger, A. flavus, A. flavus parasiticus, Penicillium citreonigrum, Acremonium sp., Alternaria alternata, Curvularia Iunata, Fusarium oxysporum, F. solani, Rhizopus stolonifera and Scopulariopsis fimicola were isolated and identified. The cropping period during April 2020 to October 2020 the moisture content, temperature and relative humidity varied from 74 to 75%, 26.9 to 36.6 °C and 73 to 88%and the spawn run during the rest of the month varied from 19 to 21 days. The maximum time of spawn run was recorded at 21 days in the month of October 2020 and minimum time of spawn run was recorded at 20 days in the month of July and August 2020.