Deciphering the Genetic Identity and Fidelity of Banana Genotype Musa acuminata through Molecular Fingerprinting

Abstract

Micropropagation for extensive production of elite planting materials is an effective and superior alternative to suckers and conventional means of propagation of Musa spp. The present investigation was carried out to evaluate the genetic identity of different micropropagated banana genotypes Grand Naine, Shrimantee and Basrai. Further micropropagated banana genotypes Grand Naine comparing with mother plant during different stages of subculture using Simple Sequence Repeat (SSR) and Inter Simple Sequence Repeat (ISSR). Moreover, for micropropagation explants (suckers) were inoculated on initiation medium containing MS (Murashige and Skoog) supplemented  with 5 mg/l BAP (Benzyl amino purine) and 39 mg/l AdSO4 (Adenine sulphate). The second, third and fourth subcultures were carried out to separate all multiple shoots. Furthermore, for rooting MS media fortified with growth hormones viz., 2 mg/l BAP, 1mg/l IBA (Indole-3-butyric Acid) and 200mg/l activated charcoal were used under present investigation. Findings revealed that 90 % and 75 % plantlets were successfully established during primary hardening in controlled and natural field condition. Further, 26 primers were screened from that 20 primers (ISSR and SSR) showed 146 clear, reproducible and scorable bands ranging from 100-2000 base pairs produced. The banding pattern of these randomly selected plants was monomorphic with mother plant of all selected genotype that confirmed the clonal uniformity.