Micropropagation and Production of Rondeletia odorata Jacq from In vitro Technique

Abstract

The objective of this study was to investigate the most suitable treatments for micropropagation of Rondeletia odorata Jacq. Rondeletia are of slow growth, and not many cultivator of plants care to give time and space to raise them. The explants were sterilized by immersion in a Clorox solution at 5, 10, 15, 20 or 25% at 20 min plus 3 drops of Tween 20 and Hg2Cl at 0.1, 0.3, 0.5 and 0.7 mg/l at 1 min. Shoot tips and auxiliary buds survived after surface sterilization were cultured on MS-strength medium (full, half and quarter salt strength), supplemented with NAA at 0.0, 0.1, 0.5 and 1.0 mg/l and their combination. For multiplication stage, 2iP at 0.0, 0.5, 1.0, 2.0 3.0 or 4.0 mg/l and IBA at 0.0, 0.5, 1.0, 1.5 and 2.0 mg/l. The second experiment BA at 0.0, 0.5, 1.0, 1.5 and 2.0 mg/l and Kin at 0.0, 0.5, 1.0 and 1.5 mg/l were used. Shoot was cultured on MS-strength medium at full, half or quarter salt strength with activated charcoal at 0.0 or 3.0 g/l and different concentrations of NAA at 0.0, 0.1, 0.5 or 1.0 mg/l. In the same time, IBA and IAA at 0.0, 0.1, 0.5, or 2.5 mg/l was added alone for rooting. All rooted shoots were acclimatized on peat moss and sand with 1:1, 1:2 or 1:3 (v/v) under plastic tunnel under plastic house conditions. Study results, 15 % Clorox plus 0.5mg/l Hg2Cl gave good results for surface sterilization. For the explants were collected gradually from the mother plants at establishment stage, the shoots were tallest at August and June. MS at half strength and 0.5 mg/l NAA induced the highest shoot length and number of leaves in establishment stage. In the multiplication stage at the first experiments, the highest shoot length and number of leaves was recorded on MS medium supplemented with 2.0mg/l IBA. At the second experiments, the highest number of shoots was found at 1.5mg/l BA. The highest number of roots; root length and callus formation was formed at quarter strength of MS medium and 0.5mg/l NAA. A high percentage of plant survival (22.22%) was achieved at pots containing only peat moss. This study was achieved to the most suitable protocol for micropropagation of Rondeletia odorata Jacq. This Acclimatization, however, need further work to increase establishment at greenhouse.