Biological Synthesis, Characterization and Antimicrobial effect of Silver Nanoparticles (Ag-NPs) using Aqueous Extract of Mango Pulp (Mangifera indica)

Abstract

The green synthesis of silver nanoparticles proceeds through the reduction of silver ions by the phytochemicals as an initial step in the formation of the nanoparticles. The phytochemicals also involved in the subsequent steps by stabilization and directing the shape and size of nanoparticles. In this study, a mango pulp extract was used for the biosynthesis of silver nanoparticles (Ag-NPs) using “One pot biological method of synthesis” under ambient temperature. The biosynthesized silver nanoparticles were characterized through visual development of color,UV-VIS spectroscopy and Fourier transform infrared ray. The antimicrobial activities of the synthesized mango pulp Ag-NPs were determined using agar well diffusion method, MIC and MBC methods. The biosynthesized Ag-NPs showed a yellowish-brown color. Broad bell-shaped range bend was gotten from UV–Vis examination with different metabolites of MPAgNPs, this makes the plasmon band wide. Surface plasmon reverberation (SPR) of silver happens at 350 - 375 nm for the 7Nps at 2Mm concentration and 13Nps at 1Mm. The FTIR shows absorbance at 3335 m-1, 3324 m-1, 326 8 m-1, 3258 m-1, and 1640 m-1 were obtained for mango pulp extract-mediated (Ag-NPs), which indicated that proteins were the capping and stabilizing molecules in the biogenic synthesis of (Ag-NPs). Silver nanoparticles at various concentration of AgNO3 (2 mM, 1 mM, and 0.5 mM) have shown a profound effect by inhibiting the growth of E. Coli and S. Aureus with an inhibition zone of 12±0, 11.5±0.70, 11.33±1.5 and 12.5±2.12, 12±1.14, 12±4.24 using gentamycin as control (15.16±0.76. and 26.67±2.1) respectively, also MIC and MBC result of the MPAg-NPs extract have shown a –ve results confirming the potentiality of the extract against microbial forms. In conclusion, mango pulp silver nano particles demonstrated the feasibility of eco-friendly biogenic synthesis of Ag-NPs from a reliable, safe and available material (mango) that can be used for the green synthesis of Ag-NPs. And it also exhibits significant antimicrobial activity against gram –ve and gram +ve bacteria.