Antibacterial Activities of Streptomyces collinus and Streptomyces refuineus from Cocoa, Orange and Timber Plantation Soils in Akure Metropolis

Abstract

Study was carried out to determine the antibacterial activities of Streptomyces from two plantation soils in Akure metropolis against some pathogenic bacteria. Purification of the Streptomyces filtrates was done by column chromatography using chloroform and methanol in the ratio of 2:1 (v/v). The antibacterial activities of crude and partially purified metabolites were evaluated against typed and clinical cultures (human and plant) of some pathogenic bacteria. For control experiment, antibiotic sensitivity pattern of the pathogenic organisms was determined by using sensitivity disc. Streptomyces collinus ORFUTA was the most effective in inhibition of pathogenic bacteria for both crude and purified metabolite. The functional groups present in the bioactive compound of             S. collinus ORFUTA revealed structure resembling hydroxyl group, alkane group and carbonyl groups. Partially purified S. collinus ORFUTA showed the highest zone of inhibition (21.31 mm) against Staphylococcus aureus (ATCC 25923) while the least zone of inhibition (3.77 mm) was observed against Pseudomonas aeruginosa. The minimum inhibitory concentration (MIC) of the test isolates ranges from 3.75 to 15 mg/ml. Ofloxacin had the highest activity 28.00 mm against S. aureus. Streptomyces collinus from orange plantation soil from Federal university of Technology Akure can be exploited in antibiotics production to treat staphylococcal infection.