Bioremediation of Polycyclic Aromatic Hydrocarbons by Bacillus Licheniformis ATHE9 and Bacillus Mojavensis ATHE13 as Newly Strains Isolated from Oil-Contaminated Soil
Somayeh Eskandari, Mehran Hoodaji, Arezoo Tahmourespour, Atousa Abdollahi, Tahere Mohammadian Baghi, Saeid Eslamian, Kaveh Ostad-Ali-Askari
Journal of Geography, Environment and Earth Science International · pp. 1–11 · Published 4 Aug 2017
10.9734/JGEESI/2017/35447Abstract
Removal of Polycyclic Aromatic Hydrocarbons (PAHs) from the environment is important because of their potentially deleterious affect human health. Biological methods that was applied in the present study is typically contain microbial processes. It is the most innocuous and effective solution that transform pollutant to nontoxic or less toxic substances. The objectives of the present study are enrichment, isolation and identification of PAHs degrading bacteria from oil-contaminated soil by molecular detection methods. Eight indigenous bacteria were isolated from oil-contaminated soils by using the enrichment methods. Two isolates with the highest optical density potentials were selected in media with 12.8 mg. L-1 of 16 PAHs as the sole source of carbon. They were identified as Bacillus licheniformis ATHE9 and Bacillus mojavensis ATHE13 by subjected to polymerase chain reaction with 16SrDNA primer and biochemically characterized. The bacterium strain including Bacillus licheniformis ATHE9 was isolated and removed by Acenaphtylene, Acenaphtene and Indeno pyrene in 72, 96 and 96 hours, respectively. The Bacillus mojavensis ATHE13 could remove Naphthalene in 72 hours and Acenaphtene, Acenaphtylene, Benzo(ghi)prylene, Dibenzo(ah)anthracene and Indeno pyrene in 96 hours. The results of this study strongly indicate that some of the indigenous bacteria have the potential role to remediate polycyclic aromatic hydrocarbons from oil-contaminated sites. These bacterial strains were able to grow in media with 12.8 mg. L-1 of 16 PAHs and reach the stationary phase. The results showed that using native bacteria with PAH-utilizing capabilities in contaminated sites could be useful for removal of these compounds from the environment.
Cited by 20
Hawra Qays, Asia Fadhile Almansoory, Israa Abdulwahab Al-Baldawi · IOP Conference Series: Earth and Environmental Science · 2023
A.A. Pashayan, A.V. Nesterov, O.S. Shchetinskaya · Ecology and Industry of Russia · 2022
Heba A.R. Abdelhaleem, Haggag S. Zein, Abdel Azeiz · Environmental Toxicology and Pharmacology · 2019
Salim Ahmed, Khushboo Kumari, Durgeshwer Singh · Arab Gulf Journal of Scientific Research · 2023
A. Giridhar Babu, Shahi I. Reja, Nadeem Akhtar · Microorganisms for Sustainability · 2019
Suranajana Sarkar, Tina Basu, Subhamoy Banerjee · Environmental Contamination Remediation and Management · 2026
Beema Kumari, Harish Chandra, Ram Chandra · Cleaner Chemical Engineering · 2022
Tanvir Kaur, Rubee Devi, Divjot Kour · Biologia · 2021
Sumira Malik, Shristi Kishore, Shradha A. Kumar · Emerging Technologies in Applied and Environmental Microbiology · 2023
Katarzyna Wojtowicz, Teresa Steliga, Piotr Kapusta · Molecules · 2023
Related research
- Effect of Woodchips on Bioremediation of Crude Oil-polluted Soil — shares topic coverage
- Characterization of Hydrocarbon Utilizing Bacteria and Fungi Associated with Crude Oil Contaminated Soil — shares topic coverage
- Detection of Resistance Genes in Escherichia coli Isolated from Fishes and Shellfishes in Creek Road/Bonny Estuary, Port Harcourt, Nigeria — shares topic coverage
- Isolation and Identification of Bacteria from Microplastic-Polluted Soil from Three Geopolitical Zones in Osun State, Nigeria — shares topic coverage
- Analysing the Role of Environmental Stresses on Species Richness and the Process of Hierarchical Structuring of Species Abundances in Marine Gastropods communities at Suva (Fiji Islands) — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
20
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.