Rheological Properties of Erythrocytes of Healthy Piglets during the Transition from Dairy to Vegetable Nutrition

Abstract

In the course of ontogenesis mammals can experience some changes of regular elements’ micro rheological peculiarities. These changes can influence blood rheological properties in capillaries and, so, the degree of tissues’ perfusion. The aim: to determine erythrocytes’ micro rheological properties in healthy piglets of dairy-vegetable nutrition. The study used 39 healthy piglets of the breed “Large-White”. They were examined on the 21st, 25th, 30th and 40th days of life. All the piglets were kept in the pig complex “Verdazernoprodukt” in Ryazan region, Russia. We evaluated in animals the activity of lipid peroxidation in plasma and erythrocytes in terms of the number of acylhydroperoxides and malon dialdehyde in them. The content of cholesterol and total phospholipids in erythrocytes was determined. In erythrocytes the surface properties of their membranes and aggregation activity were evaluated. Statistical analysis of the results was conducted by Student’s t-criterion. Against the background of strengthening of erythrocytes’ antioxidant protection we noticed lowering of acylhydroperoxides’ quantity on 13.2% and the level of malon dialdehyde – on 19.5% in erythrocytes of piglets. During the phase of dairy-vegetable nutrition the piglets were registered to have lowering of erythrocytes-discocytes till 83.3±0.09% at little rise of the level of reversibly and irreversibly changed erythrocytes. In the course of the experiment the piglets had increase of summary level of erythrocytes’ involvement into aggregates on 20.5% and quantity rise of the aggregates themselves on 22.7% at lowering of free erythrocytes’ number on 8.6%. Thus, piglets of dairy-vegetable nutrition are characterized by optimum of the processes of lipids’ peroxidation in platelets. It is accompanied by some rise of erythrocyte aggregation and quantity increase of reversibly and irreversibly changed erythrocytes in blood.