Antibacterial Activity of Vitex negundo L. against a Multidrug Resistant Pathogenic Bacterium

Abstract

Aims: The aim of this present study is to investigate antibacterial activity of extracts and/or essential oils of Vitex negundo L. leaves to an unknown pathogenic bacterium have been resistant to various groups of antibiotics Study Design: The study was design followed by previously studied and manuals of antimicrobial susceptibility test. Methodology: For antimicrobial susceptibility test disks diffusion method were used according to the guideline of European Committee on Antimicrobial Susceptibility Testing followed by McFarland standards. Various groups of antibiotics were used for conforming resistant of this unknown pathogen. The zone of inhibition and MIC was compared and measures with National Committee for Clinical Laboratory Standards (NCCLS) guidelines and reference data of previously studied. Results: According to NCCLS and data to our study revealed that UB201201 (unknown) was a multidrug resistant bacterium. Molecular identification discloses the bacterium were Bacillus cereus group. All extracts of Vitex negundo L. leaves (ethanol, chloroform, ethyl acetate and hexane) and essential oils were responsible for antibacterial susceptibility with inhibition zone of 29±0.7, 9±0.5, 8±0.3, 12±0.7, and 25±0.2 mm respectively. Conclusion: This study signifies ethanolic extract of Vitex negundo L. leaves as moderate antimicrobials and can be used for pharmaceutical and medicinal purpose. Therefore, isolation and identification of bioactive compounds from this plant will be an interest for human being.