Isolation and Characterization of Phosphate Solubilizing Bacteria from the Rhizosphere of Faba Bean (Vicia faba L.) in Meknes Region, Morocco
Abderrazak Rfaki, Laila Nassiri, Jamal Ibijbijen
Microbiology Research Journal International · pp. 247–254 · Published 28 Jan 2015
10.9734/BMRJ/2015/14379Abstract
Aims: The objective of this research was to isolate phosphate solubilizing bacteria (PSB) from faba bean rhizosphere in Meknes region and evaluate in vitro their potential for P solubilization as well as their response to the effect of temperature, NaCl, pH and antibiotics. Study Design: Rhizosphere soil and root nodules from faba bean crop in different sites from Meknes region were collected for the study. Place and Duration of Study: Department of Biology (Soil & Environment Microbiology Unit) Faculty of Sciences, Moulay Ismail University, Meknes, Morocco; between February and August 2014. Methodology: Four strains, which retained their P-solubilizing ability after repeated subculturing, from a total of seventy eight isolates were purified, characterized and identified by 16S rRNA gene sequencing. Results: The isolates were closely related to Pseudomonas cedrina (PT19), Rahnella aquatilis HX2 (P24), Rhizobium nepotum (BSP30) and Rhizobium tibeticum (Rh8). P solubilization index of these isolates ranged from 1, 57-2, 88 in tricalcium phosphate amended National Botanical Research Institute's Phosphate (NBRIP) agar medium. Amount of phosphate solubilized ranged from 62.45 to 119.95 mg P L-1 and drop in pH of the medium ranged from 7 to 3.5. Besides, these strains exhibited resistance to several antibiotics. Conclusion: The ability of the PSB to solubilize insoluble P and their adaptation to environmental conditions add value to these strains, which could lead to improved inoculants to increase the available phosphorus in the soil for faba bean plant growth and to promote sustainable agriculture.
Cited by 34
K. Bencherif, F. Senagra, L. Smain · Symbiosis · 2026
Heba Abdel-motaal, Amro Abdelazez, Shaima Abozaed · Applied Biochemistry and Biotechnology · 2026
Etsay Mesele, Ameha Kebede, Y. T. Redda · Discover Agriculture · 2025
Kaoutar Nassef, Abdelouahab Sahli, S. Bouhdid · Geomicrobiology Journal · 2025
Khadijattou Taoufiq, Laila Aberchane, Oukacha Amri · International Journal of Plant Biology · 2024
H. K. Mondal, R. Gera · Agriculture, Ecosystems & Environment. Applied Soil Ecology · 2024
S. Hadian, Donald L. Smith, Stanislav Kopřiva · Plants · 2024
Osuyi Gerard Uyi, B. Sani, O. Orole · Lafia Journal of Scientific and Industrial Research · 2024
Yang Li, Ze-Miao Liu, Lijin Yang · Horticulturae · 2023
Shumaila Batool · THE JOURNAL OF MICROBIOLOGY AND MOLECULAR GENETICS · 2022
Related research
- Screening, Growth Characterization and Alkaline Phosphatase Potential of Pseudomonas plecoglossicida from Mangrove Soil — shares topic coverage
- Toxicity of Herbicide, Paraquat Dichloride and Insecticide, Lambda-cyhalothrin on Phosphate Solubilizing Bacteria, Pantoa dispersa in Aquatic Ecosystems — shares topic coverage
- Exploring the Hidden Treasures: Isolation, Characterization and Screening of Phosphate Solubilizing Bacteria (PSB) for Plant Growth-Promoting Traits from Chilli Field Weeds Rhizosphere — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
34
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.