Molecular Characterization and Antibiotic Resistance Profile of Bacteria Associated with Clarias gariepinus (Burchell, 1822) From Ebute Iboro Location on Yewa River

Abstract

Bacterial isolates associated with Clarias gariepinus were collected from fish samples gotten on Yewa River in Ebute Iboro. The isolates were assessed using 16S rRNA gene sequencing method to identify the strains and to construct the phylogenetic relationship. The morphometric characteristics such as weight, standard length, total length and head length of fish samples were measured. Bacteria were isolated from the gills, skin and gut of Clarias gariepinius using spread plate method. The DNA of the bacteria isolates were extracted using CTAB method. PCR amplification of 16S ribosomal RNA gene of isolates was carried out using universal primer for bacteria, purification of the PCR product using ethanol precipitation, thereafter sequenced using an automated DNA sequencer. The homologous of nucleotides was then searched at the gene bank of NCBI and the isolates were identified. The antibiotics sensitivity profile of the bacterial isolates was determined using disc diffusion method. Three main types of bacteria. Pseudomonas spp, Serratia sppand Aeromonas spp, were found dominantly on the skin. The sensitivity test showed that the identified bacterial isolates were sensitive to Ofloxacin (5 mg), Nitrofurantoin (300 mg) and Ciprofloxacin (5 mg) which can be used to treat the bacteria isolate to reduce its pathogenic impact on human and to reduce disease outbreak when cultured. The bacterial isolates were 100% resistant to Cefixime (5 mg) and Cefuroxime (30 mg).