Isolation and Identification of Orange M2R and Green GS Dye Decolourizing Bacteria from Textile Sludge (Soil) Samples and Determination of Their Optimum Decolourization Conditions

Abstract

Objectives: Azo dye accounts for majorly produced synthetic dye substances in industries, posing a threat to all possible life forms. This study was focused to isolate azo dye “Orange M2R” and “Green GS” degrading bacterial strain from textile effluent soil samples and optimization of their optimum physio-chemical growth conditions. Methodology: To achieve above-mentioned objective, sludge samples were collected from textile industrial area and were applied to 1%, 3% and 5% dye containing SM broth to observe the dye degrading capability of those samples that contain acclimatized bacteria. ABIS microbiology software (Advanced Bacterial Identification Software) was used to justify and determine the identity of these bacteria with the aid of results obtained from the biochemical tests that were undertaken. Results: Bacterial strains identified in this study were Enterococcus termitis, Enterococcus camelliae, Bacillus farraginis, Bacillus muralis, Paenibacillus macerans, Bacillus decolorationis, and Macrococcus brunensis. Out of these isolates Enterococcus termitis, Bacillus farraginis, Paenibacillus macerans, Bacillus decolorationis emerged out to be most potent decolourizer, being selected for further studies. Bacillus farraginis was identified as the best decolourizer of OM2R (Orange M2R) dye that decolourized 98% of the dye and Paenibacillus macerans showed maximum decolourization on GGS(Green GS) dye that decolourized 97% of the dye. The effect of pH, NaCl, temperature and initial concentration of dye was studied with an aim to determine the optimal conditions required for maximum decolourization. The research showed different decolourization rate with varying parameters. The optimum pH for decolourization of OM2R and GGS dye was 7.0, the optimum NaCl concentration for decolourization was 2%, initial dye concentration was 1% and the temperature was 37°C for optimum decolourization by the selected isolates. Conclusion: The findings are well acclimatized and have potentials for bioremediation in textile waste effluent treatment plants.