Skip to content
Research Article Open access CC BY 4.0

Occurrence of Heavy Metal and Antibiotic Resistant Bacteria in Soils from Selected Mechanic Workshops in Ibadan Metropolis, Nigeria

Omotayo Sindiku, Omolola Oluboyede, Victor Oripenaye, Felicia Adesina, Ademola Adelekan

Journal of Scientific Research and Reports · pp. 104–116 · Published 5 Sep 2022

10.9734/jsrr/2022/v28i1030563

Abstract

Aim: This study investigated the occurrence of antibiotic and heavy metal co - resistance in bacteria indigenous to mechanic workshops. Study Design: The study is an experimental study. Place: Samples were collected at mechanic workshop. Methodology: Soil samples were collected from three mechanic workshops and tested for the presence of heavy metals. Hydrocarbon-utilizing bacteria was also isolated from the soils using Bushnell Haas medium supplemented with 1% engine oil or petrol. Isolates obtained were subjected to antibiotic resistance test and those that showed extensive drug resistance to the tested antibiotics were further tested for heavy metal resistance. Selected isolates were then identified using 16S rRNA gene sequence. Results: The soil samples contained excessive amounts of Lead, Iron, Copper and Zinc, while the concentrations of Nickel, Cobalt, Cadmium and Chromium were within WHO permissible limit. A total of 10 hydrocarbon-degrading isolates were obtained from the soil samples, seven of which were gram negative and three were gram positive. Three of the isolates showed extensive drug resistance to 14 of the tested antibiotics. Three isolates were then subjected to heavy metal resistance test and all of them showed resistance to the tested heavy metals. They were identified and given ascension numbers as Stenotrophomonas maltophilia MW392903, Achromobacter xylosoxidans MW392904 and Pseudomonas aeruginosa MW392905. Conclusion: It was observed that heavy metal resistance and antibiotic resistance can be selected for simultaneously as organisms adapt ways to cope with man’s activities in the environment, and while traits like hydrocarbon utilization and heavy metal resistance make the organisms promising in bioremediation, the inadvertent possession of antibiotic resistance by these environmental isolates poses a challenge to the health sector.

Heavy metals resistance antibiotic resistance Stenotrophomona smaltophilia Achromobacter xylosoxidans Pseudomonas aeruginosa

References (41)

  1. 1 Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically
  2. 2 Environmental monitoring and assessment [DOI]
  3. 3 Microbial heavy-metal resistance [DOI]
  4. 4 Efflux-mediated heavy metal resistance in prokaryotes [DOI]
  5. 5 Advances in the biosorption of heavy metals [DOI]
  6. 6 Microbial Population Changes during Bioremediation of an Experimental Oil Spill [DOI]
  7. 7 Exploiting the genetic and biochemical capacities of bacteria for the remediation of heavy metal pollution [DOI]
  8. 8 Review on Remediation Technologies of Soil Contaminated by Heavy Metals [DOI]
  9. 9 Identification of the pharmaceuticals for human use contaminating the Italian aquatic environment [DOI]
  10. 10 Heavy Metals Contamination and what are the Impacts on Living Organisms [DOI]
  11. 11 Bacterial Succession in a Petroleum Land Treatment Unit [DOI]
  12. 12 Influence of Traffic Activity on Heavy Metal Concentrations of Roadside Farmland Soil in Mountainous Areas [DOI]
  13. 13 Metal resistance in bacteria [DOI]
  14. 14 Influence of Petroleum Hydrocarbon Contamination on Microalgae and Microbial Activities in a Long-Term Contaminated Soil [DOI]
  15. 15 Determination of Heavy Metal Pollution in Grass and Soil of City Centre Green Areas (Konya, Turkey)
  16. 16 Profile of Heavy Metals from Automobile Workshops in Akure, Nigeria [DOI]
  17. 17 Evaluation of heavy metals in soils around auto mechanic workshop clusters in Gboko and Makurdi, Central Nigeria [DOI]
  18. 18 Development of a molecular method for detection and identification of Xanthomonas campestris pv. viticola [DOI]
  19. 19 Resistance of Bacteria Isolated from Otamiri River to Heavy Metals and Some Selected Antibiotics
  20. 20 Biological Monitoring of Cadmium [DOI]
  21. 21 Assessment of soil-pollution by slag from an automobile battery manufacturing plant in Nigeria [DOI]
  22. 22 Chemical Speciation and Mobility of Some Heavy Metals in Soils around Automobile Waste Dumpsites in Northern Part of Niger Delta, South Central Nigeria [DOI]
  23. 23 Properties of Soils and Plants Uptake within the Vicinity of Selected Automobile Workshops in Ile-Ife Southwestern, Nigeria [DOI]
  24. 24 Soil pollution in two auto-mechanic villages in Benin City, Nigeria. [DOI]
  25. 25 Assessment of Heavy Metal in Soil due to Human Activities in Kangar, Perlis, Malaysia
  26. 26 Impact of Effluents from a Car Battery Manufacturing Plant in Nigeria on Water, Soil, and Food Qualities [DOI]
  27. 27 Bioremediation potential of three acclimated bacteria with reference to heavy metal removal from waste
  28. 28 Isolation and Identification of Hydrocarbon Degradation Bacteria and Phosphate Solubilizing Bacteria in Oil Contaminated Soil in Bojonegoro, East Java, Indonesia [DOI]
  29. 29 HEAVY METAL CONTAMINATION AND PHYSICOCHEMICAL CHARACTERISTICS OF SOILS FROM AUTOMOBILE WORKSHOPS IN ABRAKA, DELTA STATE, NIGERIA
  30. 30 Soil bacterial flora and enzymatic activities in zinc and lead contaminated soil
  31. 31 Isolation and characterization of heavy metals resistant bacteria from Lagos Lagoon [DOI]
  32. 32 Antimicrobial Resistance of Salmonella enterica Serotype Typhi in Dakar, Senegal [DOI]
  33. 33 Study of Heavy Metal Speciation in Sediments Impacted with Crude Oil in the Niger Delta, Nigeria [DOI]
  34. 34 Assessment of direct soil pollution in automobile junk market [DOI]
  35. 35 Assessment of the Effects of Wood Processing Industries in Selected Parts of Delta State, Nigeria, On the Soils and Vegetation in Their Vicinities [DOI]
  36. 36 International Journal of Environmental Research and Public Health [DOI]
  37. 37 African Journal of Environmental Science and Technology [DOI]
  38. 38 International Journal of Environmental Science and Technology [DOI]
  39. 39 Archives of environmental contamination and toxicology [DOI]
  40. 40 International Research Journal of Public and Environmental Health [DOI]
  41. 41 Battling Resistance to Antibiotics and Pesticides: An Economic Approach [DOI]

Cited by 5

The chemical design of antimicrobial ionic liquids guided by machine learning: a review on balancing efficacy and toxicity

Zheng Liu, Qiuyu Chen, Congfei Yao · Journal of Materials Chemistry B · 2026

Kosakonia arachidis: Shielding Vigna radiata in metal-stressed Hydroponics

Nidhi Saini, Sumer Singh, Parul Chowdhury · Environment Conservation Journal · 2024

An Analysis of Heavy Metal Contamination in the Vicinity of Cassava processing mills in Aba, Abia State

Department of Microbiology, Federal University Gusau, Zamfara State, Nigeria., Okoye Rosemary, Amechi S. Nwankwegu · Current Research Bulletin · 2025

Showing 3 of 5 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

53

Page views

0

PDF downloads

47

Outbound clicks

5

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.