Genetic Diversity Study of Cinnamomum cassia Blume Using Random Amplified Polymorphic DN A Markers (RAPD)

Abstract

Aims: To investigate Genetic diversity study of Cinnamomum cassia Blume in Vietnam. Methodology: Fresh leaves of Cinnamomum cassia Blume sample were grinded in liquid nitrogen and total genomic DNA was extracted and purified by CTAB method. The primer was amplified via the polymerase chain reaction on a Gene Amp PCR system 9700. The binary data set was utilized to compute the pairwise Jaccard similarity index and to construct the related similarity matrix. All analyses were performed by using the NTSYS-PC software application. Results: Twenty-four accessions of Cinnamomum cassia Blume belongs to Cinnamomum species collected from some mountainous areas of the Yenbai province, Vietnam were examined to assess genetic variation using the RAPD method. The accessions included 4 in Lucyen, 5 in Tranyen, 5 in Vanchan, 6 in Vanyen and 4 in Yenbinh. Eighty-one out of 99 bands detected by 15 selected RAPD primers were polymorphic. The average number of band/primer was 6.6, in which the polymorphic bands/primer was 5.4, corresponding to 82 %. The similarity indexes among accessions fluctuate between 0.67 to 0.91 The coefficient of estimated PIC/primer range from 0.371 to 0.883, with an average of 0.756. The hight polymophylic and similarity indexes showed large modification of genome. Grouping accessions in phylogenetic tree reflected the fact that the different areas may used the same or different cultivars of Cinnamomum cassia Blume for cultivation. Conclusion: Cinnamomum cassia Blume belongs to Cinnamomum species was introduced in Yenbai from multiple sources and possesses hight genetic diversity and variation and suggests that necessary to establish conservation plan, evaluation and selection of the best gene resources for commercial purposes.