Clinico-hematological Effects of Nanosilver Administration in Wistar Rats

Abstract

This study evaluated the haematological effects of the oral administration of silver nanoparticle in Wistar rats. A total of 35 rats, six weeks of aged, both male and female, were randomly divided into two groups. Group 1 was kept as control and comprised of 20 rats. Group II comprised of 15 rats and the rats were orally administered silver nanoparticle mixed in distilled water at no-observed-adverse-effect level (NOAEL) dose rate of 30 mg/kg body weight/day daily for 90 days of experiment. Blood was collected from 5 rats from each group at 0, 30th, 60th and 90th days post-treatment. A traditional Manual method was used to measure Hematological parameters and data analyzed by one-way ANOVA (post hoc Dunnett's test). The hematological parameters studied were significantly altered in treatment group as compared to control animals. Morphology of erythrocytes indicates normocytic hypochromic anaemia. Our results indicated that the silver nanoparticles have adverse effects on general clinical condition and hematological parameters of rats at NOAEL dose.