Parental Polymorphism and Hybrid Testing in Linseed (Linum usitatissimum L.) by Using Molecular Markers

Abstract

Linseed (Linum usitatissimum L.) is a valuable oilseed crop known for its nutritional and industrial significance. Despite its importance, linseed yield remains suboptimal in India due to limited genetic advancement. This study aimed to assess parental polymorphism and confirm hybrid purity using Random Amplified Polymorphic DNA (RAPD) markers. A linseed hybrid (FR.111 × LSL.93) and its parental lines, including a dwarf genotype, were evaluated both morphologically and molecularly. Variation in characteristics like plant height, days to blooming, and seed output per plant were found by morphological analysis, suggesting heterosis in the F₁ generation. DNA was isolated by using CTAB method and amplified with five RAPD primers. Electrophoresis of PCR products generated clear banding patterns that were used to assess genetic similarity and hybridity. The presence of parent-specific bands in F₁ individuals confirmed the hybrid's genetic authenticity and purity. The study demonstrated the effectiveness of RAPD markers in detecting polymorphism and verifying hybrid identity. These findings highlight the utility of molecular markers in linseed breeding programs, offering a rapid and reliable method for hybrid confirmation. Integration of marker-assisted selection (MAS) can significantly enhance the efficiency of linseed improvement and support the development of high-yielding, genetically diverse cultivars.