Promotor Analysis of Cattle Endometrium throughout Oestrous Cycle and Early Gestation Period

Abstract

Endometrial gene expression is principally controlled by the ovarian steroids and gestation recognition features. An important number of analyses of differential expression genes (DEGs) in bovine endometrium have been reported. Bovine uteri at follicular phase (FS), luteal phase (LS) and implantation phase (IS). A promoter, as associated to genomics, is a section of DNA upstream of a gene where applicable proteins (such as RNA polymerase and transcription factors) bind to inductee transcription of that gene. The subsequent transcription produces an RNA molecule (such as mRNA). RNA polymerase and the basic transcription factors (TFs) bind to the promoter sequence and start transcription. TFs, belong to growing family of regulatory protein, influence transcription by regulating many different cellular function by interacting directly with DNA. 1kb upstream of the promoter region of each DEGs analyzed by NSITE (Recognition of Regulatory motifs) of Softberry (http://www.softberry.com) and predicated several TFs binding site. GO (gene ontology) used for identification of those DEGs have TFs functions. Promoter analyses predicted 150-160 TFs for each phase. DLX4 and IRF4 at FS, and IRF5, IRF9, STAT1 and STAT2 at IS were in common to DEGs and estimated TFs, respectively. The current research identified possibility molecular mechanisms governing inner epithelial role and coming events studies.