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Research Article Open access CC BY 4.0

Phytochemical Constituents of Carrot (Daucus carota) Fruit Juice and its Hepatoprotective Property in CCl4-Induced Liver Cirrhotic Rats

Asiat Na’Allah, Mutiu Adewunmi Alabi, Fatai A. Kareem, Ayodeji O. Obatoye, Shukuriyya Attahir, Rahma Umar

Asian Journal of Research in Biochemistry · pp. 40–55 · Published 13 Aug 2021

10.9734/ajrb/2021/v8i430189

Abstract

Aim: This study evaluated the phytochemical constituents of Carrot fruit juice (CFJ) and its hepatoprotective property in CCl4-induced liver cirrhotic rats. Study Design: Sixty male rats of weight ranging from 150-180 g were completely randomized into six groups. All rats were administered 0.5 ml/kg CCl4 subcutaneously thrice weekly except groups 1, 2, 3, and 4 while rats in groups 3 and 6 and groups 4 and 5 orally received 2.5 and 5.0 ml/kg of CFJ on daily basis for 12 weeks. Results: The preliminary qualitative phytochemical screening of extract revealed the presence of alkaloids, flavonoids, cardiac glycosides, carbohydrate, saponin, phenolic compound and tannins. The extract treated groups significantly revealed an increase in liver cirrhotic emaciated body weight and reduction in the liver index, a reversal of liver marker enzymes activities, an increase in enzymic and non-enzymic antioxidants with a decrease in malondialdehyde level reduction in C-reactive protein, interleukin-6, alpha-fetoprotein, and carcinoembryonic antigen. Exposure of animal to CCl4 induces oxidative stress, increases the generation of reactive oxygen species and myeloperoxidase activity, and reduces cell viability but was reversed by the CFJ. Conclusion: The result showed that CFJ is a promising therapeutic option for treating liver failure.

Oxidative stress reactive oxygen species myeloperoxidase activity cell viability inflammation

References (28)

  1. 1 Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays [DOI]
  2. 2 Free radicals and antioxidants in normal physiological functions and human disease [DOI]
  3. 3 Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase.
  4. 4 Colorimetric assay of catalase [DOI]
  5. 5 Antimicrobial activity of flavonoids [DOI]
  6. 6 Molecular Regulation of Hepatic Fibrosis, an Integrated Cellular Response to Tissue Injury [DOI]
  7. 7 Xanthine oxidase and neutrophil infiltration in intestinal ischemia [DOI]
  8. 8 Myeloperoxidase-generated oxidants and atherosclerosis [DOI]
  9. 9 Postulated Carbon Tetrachloride Mode of Action: A Review [DOI]
  10. 10 Mitochondrial and nonmitochondrial reduction of MTT: Interaction of MTT with TMRE, JC‐1, and NAO mitochondrial fluorescent probes [DOI]
  11. 11 Spectrophotometric assays for total antioxidant capacity (TAC) in dog serum: an update [DOI]
  12. 12 Evidence of Hepatocyte Apoptosis in Rat Liver after the Administration of Carbon Tetrachloride [DOI]
  13. 13 Antioxidant Activities of Grape ( Vitis vinifera ) Pomace Extracts [DOI]
  14. 14 C-reactive protein: a nontraditional serum marker of cardiovascular risk [DOI]
  15. 15 Fucoidan ameliorates steatohepatitis and insulin resistance by suppressing oxidative stress and inflammatory cytokines in experimental non-alcoholic fatty liver disease [DOI]
  16. 16 Noninvasive measures of oxidative stress status in humans [DOI]
  17. 17 Increased procollagen mRNA levels in carbon tetrachloride-induced liver fibrosis in rats. [DOI]
  18. 18 Zizyphus spina-Christi Extract Protects Against Aflatoxin B1- Intitiated Hepatic Carcinogenicity [DOI]
  19. 19 Protective effect of Lygodium flexuosum (L.) Sw. extract against carbon tetrachloride-induced acute liver injury in rats [DOI]
  20. 20 Diagnostic Value of Preoperative Serum Carcinoembryonic Antigen and Carbohydrate Antigen 19-9 in Colorectal Cancer [DOI]
  21. 21 In VivoEvaluation of Ethanolic Extract ofZingiber officinaleRhizomes for Its Protective Effect against Liver Cirrhosis [DOI]
  22. 22 The Assessment of Biological Activities Associated with the Major Constituents of the Methanol Extract of ‘Wild Carrot’(Daucus carotaL.) Seeds [DOI]
  23. 23 Risk of Betel Quid Chewing on the Development of Liver Cirrhosis: A Community-Based Case-Control Study [DOI]
  24. 24 Proliferation of Hepatocytes and Attenuation from Carbon Tetrachloride Hepatotoxicity by Gadolinium Chloride in Rats [DOI]
  25. 25 Reduction of Acute Hepatic Damage Induced by Acetaminophen after Treatment with Diphenyl Diselenide in Mice [DOI]
  26. 26 Liver Tissue Engineering [DOI]
  27. 27 Quantitative estimation of phenolic and flavonoid content and antioxidantactivity of various extracts of different parts of Plumbago zeylanica Linn
  28. 28 Yang-Gan-Wan Protects Mice Against Experimental Liver Damage [DOI]

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