Detection of Prethrombin 1 in Human Blood Plasma
Dar’ya Korolova, Volodymyr Chernyshenko, Tetiana Platonova, Tamara Chernyshenko, Eduard Lugovskoy
International Blood Research & Reviews · pp. 1–7 · Published 18 Mar 2016
10.9734/IBRR/2016/24683Abstract
Background: Prethrombin 1 is one of prothrombin derivatives which appears in the presence of thrombin in vitro. However, existence of prethrombin 1 in vivo remained questionable. The aim of present work was to detect of prethrombin 1 in vivo at abdominal aortic aneurysm, hip replacement after fracture and stroke. Methodology: Blood plasma samples of patients with abdominal aortic aneurysm, hip replacement after fracture, stroke, and patients which were treated with warfarine were collected. Detection of prethrombin 1 was completed using three independent approaches: combination of prothrombin index and ecamulin (ecarin) index; the APTT-test modified by addition of exogenous prothrombin; Western-blotting of blood plasma using polyclonal antibody to prothrombin. Results: Prethrombin 1 presence in patients’ blood plasma at studied pathologies was proven. Inactive prothrombin (descarboxy-prothrombin), but not prethrombin 1 was found in blood plasma of patients which were treated with warfarine. Developed approach can be used for testing the prethrombin 1 in clinical diagnostics. Conclusion: Prethrombin 1 at different pathologies, accompanied by coagulation disorders was detected in patients’ blood plasma directly.
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George P Kozynets, Volodymyr P Tsyhankov, Daria S Korolova · Journal of Burn Care & Research · 2021
SI “Prof. O.S. Kolomiichenko Institute of Otolaryngology of the National Academy of Medical Sciences of Ukraine”, Kyiv, T. A. Shydlovska, N. M. Voroshylova · The Ukrainian Biochemical Journal · 2025
Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, V. Chernyshenko, E. Snezhkova · The Ukrainian Biochemical Journal · 2019
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