Evaluation of Serum Levels of Endothelial Adhesion Molecules: Leukocyte Adhesion Molecule-1 (LAM-1), CD11a/CD18 (LFA-1) and CD11b/CD18 (Mac-1) in Fibrotic Patients

Abstract

Leukocyte adhesion molecule-1 (LAM-1), CD11a/CD18 (LFA-1) and CD11b/CD18 (Mac-1) are adhesion molecules and constitute important steps in the liver inflammation due to chronic hepatitis C. We measured soluble intercellular adhesion molecule (LAM-1), (LFA-1) and (Mac-1) as well as cholesterol and triglyceride concentrations in the serum of 120 patients with fibrosis. A study was carried out to analyze levels of LAM-1, LFA-1 and Mac-1in fibrotic patients, and find whether increasing with cholesterol and triglycerides. 120 serum samples from fibrotic patients were classified according to levels of Cholesterol and Triglycerides concentration into four groups. Positive LAM-1 samples were found in 90% of patients in first group, 83% in the second group, 73% in the third group and 46% in the fourth group. These levels were significantly higher than their levels in control group (p<0.0001) indicating extremely significant. Level of LAM-1in group (1) was extremely significant compared to group (4) (356 ± 70.5 vs. 209± 5 p < 0.0001 ES). High LFA-1level was found in 76% in first group, 73% in the second group, 70% in the third group, and 40% in the fourth group. The levels of MAC-1 in first group were significantly greater than their levels in control group (p<0.0001), and +ve MAC-1 samples were found in 66% in first group, 53% in the second group, 46% in third group, and 36% in fourth group. AST and ALT were significantly higher in first group, compared to healthy group (95.68 ± 33.32 vs. 31.77 ± 8.11, p < 0.001) for AST and (78.6 ± 29.86 vs. 28.55 ± 7.15 p < 0.001) for ALT indicating very significant relationship, while no significant was detected between the fourth group and healthy individuals (33.56±8.16 vs. 28.55 ± 7.15 p = 0.05 NS). Our study showed a significant increase in levels of LAM-1 and LFA-1 rather than MAC-1 in fibrosis compared to healthy individuals. The results showed the ability to circulate LAM-1 and LFA-1 to predict fibrosis disease and evaluated the relationship between circulating adhesion molecules and fibrotic patients.