Phytochemical Assessment, in vivo Hepatoprotective and Nephroprotective Evaluation of Aerva javanica Crude Methanolic Extract

Abstract

The current study was carried out to assess the effect of the crude methanolic extract of Aerva javanica for hepatoprotective and nephroprotective assessment along with their antioxidant potential against 2,2-azinobis(3-ethylenbenzthiazolin)-6sulfonic acid (ABTS) and 2,2diphenyle-1-picrylhyzyl (DPPH) using a standard protocol. Result of the crude methanolic extract of Aerva javanica showed 65.55, 59.37, 53.86, 47.49 and 34.76% inhibition against DPPH while against ABTS it showed 60.12, 48.45, 41.36, 37.99 and 31.89% inhibition at the concentrations 1000, 500, 250, 125 and 62.5 μg/mL. Ascorbic acid was used as a positive control and displayed a dose-dependent response. Results of Total phenolic content (TPC) and total flavonoid content (TFC) have shown 52% and 67%.The crude methanolic extract of Aerva javanicawas tested at two different doses for hepatoprotective and nephroprotective effect (150 and 300 mg/kg) in rabbits model. In comparison with the standard drug and normal saline, the test sample showed a significant hepatoprotective and nephroprotective effect. The effects of all the analyzed biomarkers of the liver (AST, ALT, ALP, serum triglyceride, serum cholesterol, and serum bilirubin) and Urea (Blood serum, Blood sugar, and Serum creatinine) showed significant effects both at 150 and 300 mg/kg. Both doses were found hepatoprotective (150 mg/kg and 300 mg/kg). Whereas, some liver biomarkers (ALT, ALP, serum triglyceride) of the methanolic extract at a dose of 300mg/kg showed significant hepatoprotective while some biomarkers (AST, serum cholesterol and serum bilirubin) was found effective at a dose of 150 mg/kg rather than the higher dose. The nephroprotective effect of the plant also increases in a dose-dependent manner i.e it is more effective at a dose of 300 mg/kg as compared to 150 mg/kg body weight. On the bases of these biomarkers, the plant extract was found an effective hepatoprotective at both the test doses.