Response Surface Optimization of Extraction of Polyphenols and Carotenoids from Sesbania grandiflora Leaves with Ethanol-water System
K. D. P. P. Gunathilake, K. K. D. S. Ranaweera, H. P. V. Rupasinghe, O. D. A. N. Perera, H. P. S. Jayaweera
Asian Journal of Biotechnology and Bioresource Technology · pp. 1–10 · Published 9 Dec 2017
10.9734/AJB2T/2017/38282Abstract
Response Surface Optimization of Extraction of Polyphenols and Carotenoids from Sesbania grandiflora Leaves with Ethanol-water System Aim: Response surface methodology was employed to optimize the extraction parameters for the extraction of total phenolics and carotenoids from leaves of Sesbania grandiflora with ethanol-water based system. Method: The effects of solvent concentration (30-100%), extraction temperature (30-60°C) and extraction time (30-90 min) on the recovery of total phenolics and carotenoids were investigated. Results and Discussion: The constructed models were adequate to explain the behavior of the extraction system and predict the responses, total phenolics (R2 = 88.53%) and carotenoid (R2 = 90.60) contents. The optimum extraction conditions of ethanol concentration, extraction temperature and extraction time for phenolics, were 46.6%, 70.2°C, and 110.5 min and for carotenoids, the optimum parameters were 100%, 70.2°C and 110.5 min, respectively. The optimal predicted contents for total phenolics (7.74 mg Gallic Acid Equivalent (GAE)/ g DW) and carotenoids (4.32 mg/g DW) values in the extracts were agreed with the experimental values obtained with optimum extraction conditions for each response.
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