Production of Copper Nanoparticles by Aspergillus niger and Evaluation of their Biomedical Applications
Syed Bilal Hussain, Maria Aslam, Asad Aslam, Sadaf Noor, Muhammad Zubair
Asian Journal of Biochemistry, Genetics and Molecular Biology · pp. 46–59 · Published 6 Oct 2023
10.9734/ajbgmb/2023/v15i2332Abstract
Nanotechnology is a well advanced area and different types of nanoparticles are now produced. The most revolutionary topic of the twenty-first century is nanotechnology. Recently, metallic nanoparticles (NPs) have gained a lot of attention. In this study a straightforward, safe, and economical method is used for synthesizing copper nanoparticles (CuNPs) by using cell free filtrate of Aspergillus niger. Fungi secretes enormous amount of enzymes which act as reducing as well stabilizing agents. Synthesized CuNPs were characterized by different techniques. Ultraviolet (UV)-visible spectrophotometer showed a characteristic peak at 380nm, Dynamic Light Scattering (DLS) analysis indicated that CuNPs have average size of 570nm with 0.480 PDI. SEM (Scanning Electron Microscopy) revealed CuNPs are of cubic shape. As per FTIR (Fourier Transform Infrared Analysis) two functional groups i.e., -O-H- and C=C- on the surface of CuNPs. Genetic analysis of Aspergillus niger revealed the presence of nitrate reductase enzyme that may involve in reduction of metal into NPs. Furthermore, CuNPs showed significant antibacterial action against Escherichia coli, Bacillus subtilis, Staphylococcus aureus, and Klebsiella pneumoniae with 17mm, 20mm, 17mm and 20mm respectively. CuNPs also showed antioxidant potential with 59.41µg/ml. Fungi secretes valuable enzymes with inherent ability to reduce metal ions into NPs. This reduced the cost of NPs synthesis and also synthesized NPs exhibit greater biomedical applications.
References (27)
- 1 Green Synthesis of Metallic Nanoparticles and Their Prospective Biotechnological Applications: an Overview [DOI]
- 2 Synthesis of Silver Nanoparticles Mediated by Fungi: A Review [DOI]
- 3 Rapid extraction of fungal DNA for PCR amplification [DOI]
- 4 Biological agents for synthesis of nanoparticles and their applications [DOI]
- 5 A review on metal-based nanoparticles and their toxicity to beneficial soil bacteria and fungi [DOI]
- 6 Biosynthesis of silver nanoparticles using aqueous extract from the compactin producing fungal strain [DOI]
- 7 Synthesis, Characterization, and Applications of Metal Nanoparticles [DOI]
- 8 Phytochemical screening and determination of phenolics and flavonoids in Dillenia pentagyna using UV–vis and FTIR spectroscopy [DOI]
- 9 Green synthesis and characterization of copper nanoparticles using Azadirachta indica leaves [DOI]
- 10 Fungal silver nanoparticles: synthesis, application and challenges [DOI]
- 11 Metallic Nanoparticle: A Review [DOI]
- 12 Recent advances of chitosan-based nanoparticles for biomedical and biotechnological applications [DOI]
- 13 Preparation of Chitosan Nanoparticles and their In-vitro Characterization [DOI]
- 14 New Method for Assessment of Serum Catalase Activity [DOI]
- 15 Synthesis and size control of copper nanoparticles and their catalytic application [DOI]
- 16 Biosynthesis, Characterization and Antifungal Activity of Silver Nanoparticles by Aspergillus Niger Isolate [DOI]
- 17 Gold Nanoparticles for Biology and Medicine* [DOI]
- 18 Tenebrio molitor-mediated entomopathogenic fungal library construction for pest management [DOI]
- 19 Activation of peroxodisulfate and peroxomonosulfate by green synthesized copper nanoparticles for Methyl Orange degradation: A kinetic study [DOI]
- 20 Synthesis and Characterization of PVP-Stabilized Palladium Nanoparticles by XRD, SAXS, SP-ICP-MS, and SEM [DOI]
- 21 Fungus-Mediated Nanoparticles: Characterization and Biomedical Advances [DOI]
- 22 Isolation and Identification of Endophytic Fungi from Leave and Stem of Calopogonium mucunoides [DOI]
- 23 Oxidative degradation of Orange G by peroxomonosulfate in presence of biosynthesized copper nanoparticles—A kinetic study [DOI]
- 24 Identification of Fungal Growth from the Internal Organs of Preserved Human Cadavers [DOI]
- 25 Indian Journal Of Science And Technology [DOI]
- 26 Transactions of non ferrous metals society of China
- 27 International Journal of Life-Sciences Scientific Research [DOI]
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