Profiling Rhizosphere Microbes on the Root of Maize (Zea mays) Planted in an Alfisol for Selection as Plant Growth Promoting Rhizobacteria (PGPR)
L. B. Taiwo, B. V. Ailenokhuoria, A. O. Oyedele
Microbiology Research Journal International · pp. 1–10 · Published 10 Sep 2017
10.9734/MRJI/2017/36404Abstract
Maize (Zea mays L. Merill) root rhizosphere, being a metabolite-enriched niche was profiled with the objective of isolating Plant Growth Promoting Rhizobacteria (PGPR) for bio-fertilizer production. Isolation was carried out from the root surface and rhizosphere soil samples of the plant using standard procedures. Cultural, physiological and biochemical procedures were used to identify the isolates. Eighty (80) isolates obtained were screened for growth promoting attributes and sixteen (16) representative isolates selected, and further identified using molecular methods by sequencing of their 16S rDNA gene. Sixty three percent of the characterized organisms exhibited sequence homology level equal or greater than 90% with those of the gene bank. Microorganisms with pathogenic characteristics were eliminated. As a result of this, Bacillus thuringiensis, Pseudomonas putida and Klebsiella varricola selected, were further screened in vitro for Indole acetic acid (IAA), Gibberellic acid (GA) and Cytokinin production using standard assay methods. Klebsiella varricola produced 19.697 mg/L of GA, 0.348 mg/L of IAA and 1.804 mg/L of Cytokinin. Pseudomonas putida had 2.693 mg/L of GA, 0.152 mg/L and of IAA and 5.066 mg/L of Cytokinin in solution while Bacillus thuringiensis excreted 15.091 mg/L, 0.132 mg/L and 2.410 mg/L of GA, IAA and Cytokinin respectively. The 3 PGPR are considered suitable for biofertilization programme.
Cited by 8
Neemisha Pathania, Arun Kumar, Poonam Sharma · Journal of Applied Microbiology · 2022
Maykelis Díaz Solares, Leticia Fuentes Alfonso, Madyu Matos Trujillo · Agronomía Colombiana · 2021
C. Ojesola, A. Akintokun, P. Akintokun · 2021
D. Makolo, A. Suleiman, O. Olonitola · 2020
N. Pandya, P. V. Desai, H. Jadhav · Environmental Sustainability · 2018
L. Taiwo, A. Oyedele, B. Ailenokhuoria · Sustainable Development and Biodiversity · 2019
Divjot Kour, Kusam Lata Rana, N. Yadav · Plant Growth Promoting Rhizobacteria for Agricultural Sustainability · 2019
Adedayo Omowumi Oyedele, Emmanuel Ezaka, Lateef Bamidele Taiwo · Microbial Biostimulants for Plant Growth and Abiotic Stress Amelioration · 2024
Related research
- Emerging Nutritional and Climate-resilient Strategies in Mulberry for Enhanced Cocoon Quality in Sericulture — shares topic coverage
- Indole Acetic Acid (IAA) Mediated Plant Growth Promotion Activity of Fluorescent Pseudomonads and Bacillus spp. — shares topic coverage
- Salt Tolerant Rhizobacteria from Coastal Region of Bangladesh Portrayed the Potential for Plant Growth Promotion — shares topic coverage
- Rapid Methods for Identification of Plant-Growth-Promoting Rhizobacteria — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
8
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.