Simultaneous Determination of Ternary Mixture of Aspirin, Caffeine and Orphenadrine Citrate by Simple RP-TLC Spectrodensitometric Method

Abstract

Aims: A simple TLC Spectrodensitometric method was developed for analysis of Orphenadrine Citrate (OR), Caffeine (CAF) and Aspirin (ASP) either in pure form or in their pharmaceutical preparations. Study Design: Validation study. Methodology: In this method, The three drugs were separated on silica gel plate using ethyl acetate: acetone: methanol: triethylamine (6:3:1:0.2, by Volume) as mobile phase at room temperature. Many experimental parameters such as band size, slit width, different developing mobile phases and scanning wavelengths were examined and the optimum conditions were selected. The obtained bands were then scanned at 220 nm. The three drugs were adequately resolved with the R­­f values of ASP (Rf = 0.08 ± 0.02), CAF (Rf = 0.55 ± 0.02) and OR (Rf = 0.35 ± 0.02). Validation parameters of the developed method were studied like linearity, accuracy, precision, and specificity. Results: Linearity of the proposed method was found to be in the concentration ranges 0.4-2 μg/band for ASP, 0.4-2 μg/band for CAF and 0.3-3 μg/band for OR. Conclusion: The suggested method was effectively used for analysis of ASP, CAF and OR in pure form and in their medicinal formulations. The method is proved to be specific, accurate and selective.