Standardization of In vitro Seed Culture Technique for Six Rose Varieties

Abstract

Research was conducted at Biotechnology-cum-Tissue Culture Centre under Department of Floriculture and Landscaping, College of Agriculture, Odisha University of Agriculture and Technology, Bhubaneswar, Odisha, during the year 2021-2022 to rescue the embryo of rose seeds in Six Rose varities like Pusa Baramasi, Gold Strike, Ravel, Sadabahar, Summer Fragrance and Orange Sensation with the objectives to study the effect and efficacy of plant bioregulators on In vitro germination of rose seeds. The In vitro experiment was laid out in Completely Randomized Design (CRD) with 8 treatments and 3 replications by using different combination of BAP, GA3 and AdS in MS media. The results of the study revealed that seeds of the rose variety Pusa Baramasi were the best explants for the experiment. The MS medium when supplemented with treatment T5 i.e, {BAP (2.5 mg/l) + GA3 (0.5 mg/l) + AdS (50 mg/l)} resulted in early shoot initiation in Pusa Baramasi (7.15 days) followed by Ravel (11.63 days), Gold Strike (11.89 days), Sadabahar (12.38 days), Summer Fragrance (12.95 days) and Orange Sensation (13.32 days). Maximum available days for shoot development was also observed in Pusa Baramasi (36.02 days) when MS medium supplemented with same concentration of BAP, GA3 and AdS followed by Ravel (18.17 days), Gold Strike (17.72 days), Sadabahar (17.19 days), Summer Fragrance (16.53 days) and Orange Sensation (15.85 days) in the treatment T5 significantly delayed the cessation of the growth in Pusa Baramasi (43.17 days) followed by Ravel (29.79 days), Gold Strike (29.61 days), Sadabahar (29.57 days), Summer Fragrance (29.48 days) and Orange Sensation (29.71 days) and produced maximum number of leaves in Pusa Baramasi (3.40 leaves) followed by Ravel (1.51 leaves), Gold Strike (1.50 leaves), Sadabahar (1.23 leaves), Summer Fragrance (1.07 leaves) and Orange Sensation (0.92 leaves). Pusa Baramasi showed light green leaves with the treatment T4 {BAP (2.0 mg/l) + GA3 (0.5 mg/l) + AdS (50 mg/l)} and T5 {BAP (2.5 mg/l) + GA3 (0.5 mg/l) + AdS (50 mg/l)} and other treatments showed whitish green leaves. Ravel, Gold Strike and Sadabahar varities also showed light green leaves when MS medium was supplemented with treatment T5 and rest of the treatments showed whitish green colour leaves. Summer Fragrance and Orange sensation showed whitish green colour leaves in all the treatments. Early callus initiation was observed in Pusa Baramasi (12.53 days) followed by Ravel (16.59 days), Gold Strike (17.69 days), Sadabahar (18.26 days), Summer Fragrance (18.48 days), Orange Sensation (19.21 days) and Pusa Baramasi showed light green callus when MS medium was supplemented with the treatment T5 and other treatments showed whitish green callus. Ravel, Gold Strike, Sadabahar, Summer Fragrance and Orange sensation also showed whitish green colour callus in all the treatments.