Proximate Analysis, Phytochemical Screening and Chromatographic Profiling of Cassia nodosa Ethanol Leaves Extract

Abstract

Aim: The present study was done to evaluate the nutritional and phytochemical composition of the ethanol leave extract of Cassia nodosa. Methods: Ethanol extract was prepared with powdered leaves of C. nodosa was prepared using cold extraction method. Proximate analysis was done using the AOAC method. Preliminary phytochemical screening was done using standard methods. Gas chromatography‑mass spectrometry (GC‑MS) was used for chromatographic profiling. The National Institute standard and Technology (NIST05) library was used to match and interpret the spectral. Results: The results from proximate analysis revealed that C. nodosa leaves consist of 43.59±0.66% Carbohydrate, 20.76±0.19% crude fibre, 14.20±0.55% moisture, 14.14±0.50% protein, 4.37±0.06% ash and 2.94±0.05% fat. The presence of Alkaloids, Phenol, Saponin, Tannin, Quinine, Anthraquinone, Steroids, Cardiac glycoside, Flavonoid, Diterpene,  Phenolic amino acid and Aromatic amino acid were detected in qualitative phytochemical analysis of C. nodosa. Twenty two (22) phytochemicals including 3-O-Methyl-d-glucose (53.06%), 9,12,15-Octadecatrienoic acid, (Z,Z,Z)- (3.30%), n-Hexadecanoic acid (2.73%), 8,11,14-Eicosatrienoic acid, (Z,Z,Z)- (1.68%), Spiro[androst-5-ene-17,1'-cyclobutan]-2'-one, 3-hydroxy-, (3.beta.,17.beta.)- (0.79%), 4H-Pyran-4-one 2,3-dihydro-3,5-dihydroxy-6-methyl- (0.69), and Phytol (0.51%) were identified from the GC-MS spectra gotten from ethanol leave extract of C. nodosa Conclusion: The findings of this study reveals that C. nodosa leaves are potential candidate for consumption as food as well as candidate for drug discovery