In vivo Approach: Potential Diuretic Activity of M. charantia Linn. on Alloxan Induced Albino Wistar Rats in Diabetes Mellitus

Abstract

The screening of perfect diuretics for non –clinical utility in Diabetes mellitus is a relatively novel approach which gain insight into underlying the pathophysiological processes. This study aim to evaluate the diuretic effect of crude aqueous & alcoholic extract of M. charantia Linn. using Albino Wistar Rat model. The methodology of animal study includes the spectral analysis of Na+, K+, Cl-concentration against the body weight, this was done by spectrophotometry. In this study, the comparative observation of Diuretic activity with standard and extracted compound has shown that the estimated 24‐hour urine contains the Na+-3.82 g, 3.82 g, 3.92 g  and K+-1.35 g, 1.39 g, 1.48 gwt. For Vehicle control, Standard drug, and Extracted compound respectively. Which possess the favouring result means from the spot urine were 10.7±7.0 g/24 hand 3.9±2.1 g/24 h, respectively. Coefficients were 0.035, 0.022, 0.046 at (d±2SD=7.07 g, 4.42 gand 8.92 g) for sodium chloride and 0.068, 0.031, 0.046 at (d ± 2SD = 4.92 g, 2.31 g and 3.34 g) for potassium chloride. The present study guide formulation of non-clinical trials with statistical study to further measuring the claimed efficacy of M. charantia as a natural remedy for diabetes mellitus.