Reducing Sugar, Alkaloid and Tannin from Dryopteris dilatata Fractions Modulates Diabetogenic and Oxidative Stress Activity on Alloxan Induced Diabetic Rats

Abstract

Active components in medicinal plants possess therapeutic indications in disease states. Aim: The present investigation evaluated the activity of fraction from Dryopteris dilatata leaves modulating diabetogenic and oxidative stress activity in alloxan-induced diabetic Rats. Methods: Seventy-two male (135-140)g wistar rats divided into two groups of thirty-six rats each for oral glucose tolerance test and diabetic study. Diabetic induction and oral glucose test (OGTT) was done using standard methods. Each group was divided into six sub-groups (n-6). Group A was normal control, group B diabetic control, group C received metformin 50 mg/kg, group D reducing sugar fraction 800 mg/kg, group E alkaloid fraction 800 mg/kg and group F tannin fraction 800 mg/kg once for OGTT and daily throughout the treatment period (15 days) for diabetic study. Their glucose level was taken at interval of hours for OGTT and five days interval for diabetic group. We assessed the levels of lipid profile (TC, TG, LDL and HDL), lipid peroxidation, endogenous antioxidants in the brain and testis. Results: Fractions of Dryopteris dilatata caused significant reduction in body weight and blood glucose levels in experimental rats, decreased the levels of TC, TG, LDL, increased HDL, reduced  levels of MDA, elevated CAT, GSH and SOD in the brain and testis. Conclusion: The observed results in this study connotes that fractions of Dd could be used in amelioration of diabetes and its associated complications by reducing bad cholesterol (TC, TG and LDL), increasing good cholesterol (HDL), attenuating the activity of antioxidants in diabetic condition.