Response of NaCl and Brassinolide in  Stevia rebaudiana under in vitro Condition

Ankita Singh; Padmanabh Dwivedi; Sangita Sahni; Bishun Deo Prasad

doi:10.9734/ijpss/2022/v34i2131250

Research Article Open access CC BY 4.0

Response of NaCl and Brassinolide in Stevia rebaudiana under in vitro Condition

Ankita Singh, Padmanabh Dwivedi, Sangita Sahni, Bishun Deo Prasad

International Journal of Plant & Soil Science · pp. 138–145 · Published 11 Jul 2022

10.9734/ijpss/2022/v34i2131250

Abstract

The current work sought to investigate the impact of two chemicals (NaCl and Brassinolide) on growth parameters, chlorophyll pigments in stevia shoots cultured under in vitro condition. For this, nodal explants of stevia were cultured for four weeks on MS medium supplemented with 0.2 mg l-1 BAP, Kinetin 0.2 mg l-1 and various concentrations of NaCl (0, 60, 70, 90, 120, 150 mM) and BL (0, 1, 2, 3 4, 5µM) for screening under in vitro condition. Explant treated with NaCl showed reduction with increasing salinity levels in all the studied parameters while explants treated with Brassinolide showed best results in all the studied parameters. From the results, it is concluded that the stevia plant was less impacted by 60mM and 70mM NaCl concentrations when compared to other concentrations of NaCl, but BL increased/improved all the examined parameters when compared to control; 1µM and 2µM concentrations of BL responded the best.

Brassinolide (BL) growth parameters in vitro photosynthetic pigments stress Stevia spp

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Ankita Singh, Padmanabh Dwivedi, Sangita Sahni, Bishun Deo Prasad (2022). Response of NaCl and Brassinolide in Stevia rebaudiana under in vitro Condition. International Journal of Plant & Soil Science , 138–145. https://doi.org/10.9734/ijpss/2022/v34i2131250

Affiliations

Ankita Singh — Department of Plant Physiology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi-221005, UP, India.
Padmanabh Dwivedi — Department of Plant Physiology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi-221005, UP, India.
Sangita Sahni — Department of Plant Pathology, TCA, Dholi, Dr. Rajendra Prasad Central Agricultural University, Pusa, Samastipur; Bihar-848125, India.
Bishun Deo Prasad — Department of Agricultural Biotechnology & Molecular Biology, College of Basic Sciences & Humanities, Dr. Rajendra Prasad Central Agricultural University, Pusa, Samastipur; Bihar-848125, India.

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