Antimicrobial Activity and GC-MS Analysis of Bioactive Compounds of Tribulus terrestris L.

Abstract

Aims: To examine the potential phytoconstituents present in leaves and fruits ofTribulus terrestris and its antimicrobial activity against Enterobacter cloacae. Study Design: Plant extracts preparation, determination of total phenolic content, total antioxidant activity and antimicrobial activity. GC-MS analysis Place and Duration of Study: Sample: Fresh plant parts of Tribulus terrestris were collected from the campus of Banasthali Vidyapith, Rajasthan, gram-negative bacterial strain, Enterobacter cloacaeBVDWH38 (Gene bank Acc. No. OQ772170) was used for antimicrobial study. Methodology: Plant extracts preparation; free radical scavenging of sample extract antimicrobial activity was done by standard methods. All experiments were repeated at least thrice. The data represented are Mean ± standard deviations (SD) of all the three replicates. Results: Alcoholic and aqueous leaves and fruits extracts of T. terrestris were prepared. Total phenolic compound (TPC) and antioxidant activity was determined and found to be higher in aqueous extract of both plant parts of T. terrestris.Antimicrobial activity against water borne pathogenic E. cloacaestrains was evaluated and found that aqueous extract of both leaves and fruits of T. terrestriswas more effective. Gas chromatography- Mass spectrophotometer (GC-MS) with alcoholic extract of leaves and fruits of T. terrestris identified Digitoxin (6.37%) and Ethyl iso-allocholate (22.90%) respectively among other compounds. Conclusion: The leaves and fruits of T. terrestris can be used as alternative medicine as it has vital bioactive secondary metabolites.