Single Nucleotide Polymorphism and Allele Frequencies of Some Genetic Variants among Type 2 Diabetes Mellitus (T2DM) Subjects in  Port Harcourt

Reuben, I.; Tamuno-Emine, D.G.; Nwachuku, E.O.; Bartimaeus, E.S.

doi:10.9734/ajbgmb/2024/v16i12426

Research Article Open access CC BY 4.0

Single Nucleotide Polymorphism and Allele Frequencies of Some Genetic Variants among Type 2 Diabetes Mellitus (T2DM) Subjects in Port Harcourt

Reuben, I., Tamuno-Emine, D.G., Nwachuku, E.O., Bartimaeus, E.S.

Asian Journal of Biochemistry, Genetics and Molecular Biology · pp. 100–107 · Published 19 Dec 2024

10.9734/ajbgmb/2024/v16i12426

Abstract

Aim: To evaluate Single Nucleotide Polymorphism and Allele frequencies of some genetic variants among T2DM subjects in Port Harcourt. Study Design: Cross-sectional Study. Place and Duration of Study: Port-Harcourt, Nigeria, Inqaba Biotec (molecular analysis) and Evashalom Medical Diagnostic services, between November 2022 and April 2023. Methodology: Ninety (90) subjects between aged between 18- 70years among which 54 were females and 26 males were recruited. Eight SNPs, four each from SLC22A1 and CYP2C9 genes respectively were selected and genotyped using Mass Array. Genotypic and allelic distribution employed the Hardy-Weinberg equilibrium test (HWE). The minor allele frequency (MAF) was calculated using Excel, and the Hardy–Weinberg equilibrium (HWE) test was performed using Gene-Calc software. Statistical significance was set at p < 0.05. Odd ratios were calculated using the Baptista-Pike test. Statistical significance was set at p<0.05. Results: In this study, the allelic frequency of each SNP was in HWE(p>0.05). The genotypic frequencies for rs 594709 for homozygote wild type (AA), heterozygote (AG), and homozygote (GG) were 73,33%,16.67% and 0.10% respectively. The MAF observed for rs594709 was 18.33%. The MAF observed was 18.33%. The rs72552763 variant genotype frequencies for the homozygote wild type (ATGATATGAT), heterozygote (ATGATAT) and homozygote (ATAT) were 93.33%, 6.67% and 0.0% respectively. On the other hand, the variant rs9332214 genotype frequencies for homozygote wild type (TT), heterozygote (CT), homozygote (CC) were 93.33%,6.66% and 0.0% respectively. The MAF observed for both SNPs was 0.033%. Conclusion: This study showed that the rs 594709 for homozygote wild type (AA) had the highest frequency and no polymorphism was observed in four out of the SNPs investigated.

Genetic polymorphism metformin sulphonylureas allele frequencies genetic variants T2DM subjects Port Harcourt

References (10)

1 Type 2 diabetes [DOI]
2 6. Glycemic Targets: Standards of Medical Care in Diabetes—2022 [DOI]
3 Pathophysiologic Approach to Therapy in Patients With Newly Diagnosed Type 2 Diabetes [DOI]
4 Global Prevalence of Type 2 Diabetes over the Next Ten Years (2018-2028) [DOI]
5 Genetic susceptibility to type 2 diabetes and obesity: from genome-wide association studies to rare variants and beyond [DOI]
6 Cardiovascular Risk Factor Targets and Cardiovascular Disease Event Risk in Diabetes: A Pooling Project of the Atherosclerosis Risk in Communities Study, Multi-Ethnic Study of Atherosclerosis, and Jackson Heart Study [DOI]
7 Metformin-Insulin versus Metformin-Sulfonylurea Combination Therapies in Type 2 Diabetes: A Comparative Study of Glycemic Control and Risk of Cardiovascular Diseases in Addis Ababa, Ethiopia [DOI]
8 Genetic polymorphisms and haplotypes of the organic cation transporter 1 gene (SLC22A1) in the Xhosa population of South Africa [DOI]
9 Distribution of Genetic Polymorphisms in Drug Metabolizing Gene Cytochrome P450 (CYP2C8*3 and CYP2C9*2) in a North Indian Type 2 Diabetes Population [DOI]
10 Personalized medicine for diabetes: a special issue [DOI]

Cited by 0

No indexed citations yet.

Knowledge, Attitude and Predisposing Risk Factors of Non-Albican Vulvovaginal Candidiasis among Symptomatic Women in Port Harcourt — shares topic coverage
Implanon Implant Contraception at the University of Port Harcourt Teaching Hospital: A Periodic Review — shares topic coverage
Associated Factors in the Declining Trend in the Use of Progestogen Only Injectable Contraceptive in a Niger Delta University Teaching Hospital, Nigeria — shares topic coverage
Urinary Pathogens amongst Parturients at the University of Port Harcourt Teaching Hospital, South-South, Nigeria — shares topic coverage
Assessing the HIV Knowledge, Awareness, and Utilization of YFS among Undergraduates in Rivers State, Nigeria — shares topic coverage

Access this article

View at publisher

Cite this article

Reuben, I., Tamuno-Emine, D.G., Nwachuku, E.O., Bartimaeus, E.S. (2024). Single Nucleotide Polymorphism and Allele Frequencies of Some Genetic Variants among Type 2 Diabetes Mellitus (T2DM) Subjects in Port Harcourt. Asian Journal of Biochemistry, Genetics and Molecular Biology , 100–107. https://doi.org/10.9734/ajbgmb/2024/v16i12426

Affiliations

Reuben, I. — Department of Clinical Chemistry, Faculty of Medical Laboratory Science, Rivers State University, Port Harcourt, Rivers State, Nigeria.
Tamuno-Emine, D.G. — Department of Clinical Chemistry, Faculty of Medical Laboratory Science, Rivers State University, Port Harcourt, Rivers State, Nigeria.
Nwachuku, E.O. — Department of Clinical Chemistry, Faculty of Medical Laboratory Science, Rivers State University, Port Harcourt, Rivers State, Nigeria.
Bartimaeus, E.S. — Department of Clinical Chemistry, Faculty of Medical Laboratory Science, Rivers State University, Port Harcourt, Rivers State, Nigeria.

Metrics

63
Views: 0
Downloads: 0
Citations: 15
Outbound

Full metrics

Article metrics

Real usage data collected on this platform.

63

Page views

0

PDF downloads

15

Outbound clicks

0

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.