Formulation and Evaluation of Hydroethanolic and Chloroform Extracts of Zanthoxyllum oxyphyllum for Analgesic and Wound Healing Activities

Abstract

Zanthoxylum oxyphyllum under the family Rutaceae, commonly known as Mezenga in Assam grow in tropical and temperate regions. Hydroethanolic and chloroform extracts of leaves of the plant were screened for analgesic activity and wound healing property by hot plate test and excision, incision wound model. The acute toxicity study revealed that both the plant extracts were non-toxic and safe for oral dosing. Both the extracts were non-sensitizing to rabbit skin and caused very little or no irritation in intact and damaged skin rabbits treated with single and multiple doses. Irritation scores did not differ significantly between the control and treatment groups, according to the acute dermal toxicity study. It was observed that the hydroethanolic extract of the selected plant possess significant analgesic effect against thermal stimuli and inhibits pain induced by chemically stimuli. It was also showed that hydroethanolic extract was found to be most active and was responsible for the significant and dose-dependent wound healing property comparable with the standard extract.