Standardization of Sucrose Concentration in Micropropagation of Valuable Aquascape Plant Echinodorus grisebachii

Abstract

Aims: To standardize the optimum sucrose concentration in shooting and rooting medium in micropropagation of valuable aquascape plant Echinodorus grisebachii. Study Design: CRD. Place and Duration of Study: Department of Floriculture and Landscaping, College of Agriculture Vellayani 2022-2024. Methodology: In this study, eight treatments were evaluated separately for their effectiveness in shooting and rooting medium. For the shooting medium, 2 mgL-1 BAP (6-Benzylaminopurine) and 3 sucrose concentrations (10, 20,30) gL⁻¹ were used in the MS medium and half-strength MS medium. For rooting medium 3.0 mgL⁻¹ IBA (Indole-3-butyric acid), three concentrations of sucrose as in shooting medium were tried. Results: The result of the study showed that out of 8 treatments tried for shooting medium MS + 2mgL-1 + 30 gL⁻¹ sucrose was best resulted in Shoot initiation (100 %), Minimum days to shoot emergence (6.17), Maximum number of shoots (9.83), Longest shoot (10.33) and maximum number of leaves (31). For rooting medium ½ MS + 3.0 mgL⁻¹ IBA + 30 gL⁻¹ sucrose was best resulted in minimum number of days in root emergence (12.58) and maximum root initiation percentage (99.25). Conclusion: An effective concentration of sucrose was standardized for Echinodorus grisebachii.