Studies on Physiological Effects and Plasmid Stability of JM109 Host System
Praveen Kumar Vemuri, Shaik Mohammad Anjum, Lakshmi Saranya Medida, P. V. Hemanth Sai, Mahathi Gummavajjala, Braja Kishori Panigrahi, Swati Dash, Swati Senapati
Journal of Pharmaceutical Research International · pp. 102–108 · Published 13 Nov 2020
10.9734/jpri/2020/v32i2830876Abstract
Objective: The purpose of the study delineates the growth and plasmid stability of E. coli JM109 host system. Methods: Different concentrations of drugs, chemicals and various frequency of radiations were subjected to the host system to verify the colony forming units along with plasmid concentration and stability. Results: Among chemicals, acridine orange showed highest effect on growth of DH5a, while among the drugs, dantron showed maximum effect on the growth of the organism. Radio frequency of 2GHz and low intensity microwave radiation were recorded as highest inhibitory effects. However, there is no significant effect in growth was observed in exposure to UV rays. Conclusion: The present work discussed that, the effect of drugs, chemicals, radio frequency and microwave radiation have a huge effect not only on growth of organism but also concentration and stability of plasmid.
Cited by 2
Laxmisai Dixitha Vengili, Phavethra Sago, Yaswanth Kumar Reddy Kuncha · Journal of Pharmaceutical Research International · 2021
Maheswara Reddy Mallu, Siva Reddy Golamari, Sree Rama Chandra Karthik Kotikalapudi · 2021
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