Molecular Characterization, Multiple Drug Resistance, and Virulence Determinants of Pseudomonas aeruginosa Isolated from Lebanon
Sima Tokajian, Rola Timani, Nahla Issa, George Araj
Microbiology Research Journal International · pp. 243–250 · Published 19 Dec 2012
10.9734/BMRJ/2012/2217Abstract
Aims: Typing and characterization of 100 P. aeruginosa clinical isolates by pulse field gel electrophoresis (PFGE) to detect changes in the clonal composition of local strains and to correlate banding patterns with site of infection, drug resistance and Type III secretion system effectors. Methodology: A total of 100 P. aeruginosa isolates obtained from clinical specimens were used to study resistance profiles, PFGE banding patterns and virulence determinants. Results: Results from antimicrobial susceptibility testing yielded showed that 77 of the strains were multi drug resistant (MDR). Grouping isolates as non-susceptible when tested intermediate and resistant according showed showed that resistance was 25% each for imipenem and piperacillin-tazobactam, while it was 29% for ceftazidime these drugs are among the ones most commonly used in treating infections caused by P. aeruginosa. Studying effectors released by the type III secretion system including exoU and exoS revealed that 48% of the isolates harbored exoS toxin gene and 46% the exoU, with 3% having both and 8% having none. When the different pulsotypes were compared on a dendogram, 45 groups emerged showing vast differences among the isolates. Conclusion: This study showed the emergence of drug resistance in P. aeruginosa against the antimicrobial agents being routinely used for treatment and revealed the likely presence of co-selected traits that result in highly virulent and resistant strains. Further clinical investigations are warranted to combat infections caused by this important human pathogen in Lebanon.
Cited by 14
Y. Yuandani, Abdi Wira Septama, Dinda Sari Utami · Journal of HerbMed Pharmacology · 2024
A. Gholami, D. Minai-Tehrani, S. J. Mahdizadeh · Journal of Chemical Information and Modeling · 2023
G. Ghorbani, E. Rahimi, A. Shakerian · BioMed Research International · 2022
Y. Alabdali · Gene Reports · 2021
Edit Kaszab, J. Radó, B. Kriszt · International Journal of Environmental Health Research · 2019
Pilar Gómez · Emerita · 2018
Patricia Saenz-Méndez, Martin Eriksson, L. Eriksson · ACS Omega · 2017
F. Al-Wrafy, E. Brzozowska, S. Górska · Postępy Higieny i Medycyny Doświadczalnej · 2017
N. E. El Zawawy, S. Ali · Journal of Infection in Developing Countries · 2016
N. Amirmozafari, Jalil Fallah Mehrabadi, A. Habibi · Archives of Iranian medicine · 2016
Related research
- Urine Analysis and Determination of Drug Sensitivity Pattern of Isolated Escherichia coli from Urinary Tract Infected Patients — shares topic coverage
- Alternative Strategies in the Treatment of Clostridioides difficile Infection — shares topic coverage
- Community Acquired Pneumonia in Bangladesh: Sensitivity Pattern, Clinical Courses and Outcome of a Cross Sectional Population — shares topic coverage
- Knowledge and Perception of HealthCare Workers on Antibiotic Resistance and Predictors of Antibiotic Prescription in Benue State, Nigeria — shares topic coverage
- Genotypic Analysis of Klebsiella pneumoniae among Patients Admitted in Critical Care Settings in a Tertiary Care Center of Bangladesh — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
14
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.