Effectiveness of Ethyl Acetate Extract of Endophytic Fungi in Soursop Leaves towards the Growth of Mammary Tumor Induced by 7,12-dimethylbenz(α)anthracene in Female Rats

Abstract

Aims: This research aims to investigate the effectiveness of ethyl acetate extract of endophytic fungi isolated Sir G5 from soursop leaf (Annona muricata L.) in vivo breast tumor growth using Sprague-dawley (SD) female strains induced by 7,12-dimethylbenz(α)anthracene (DMBA). Study Design: This study was an in vivo experiment using a completely randomized design   method (RAL). Place and Duration of Study: This research was conducted from July 2016 to February 2017 in Microbiology Laboratory of Biology Research Center, Indonesian Institute of Sciences (LIPI), Cibinong-Bogor, and Research Laboratory of Biochemistry Department of Bogor Agricultural University. Methodology: Twenty-four female rats were divided into six groups; normal group (administered per os (p.o) with aquadest 2 ml for twenty two weeks), negative control (-) DMBA (administered intraperitoneal (i.p.) with 20 mg/kg body weight (bw) of 7,12-dimethylbenz(α)anthracene), positive control (+) Doxo (administered i.p with 20 mg/kg bw of 7,12-dimethylbenz(α)anthracene and p.o. with 2 µg/20 0 g bw of Doxorubicin), DI (administered i.p. with 20 mg/kg bw of 7,12-dimethylbenz(α)anthracene and p.o. with extract of 20 mg/kg bw isolate Sir G5), DII (administered i.p. with 20 mg/kg bw of 7,12- dimethylbenz(α) anthracene and p.o. with 40 mg/kg bw extract of isolate Sir G5) and DIII (administered i.p. with 20 mg/kg b.w.of 7,12- dimethylbenz(α)anthracene and with 120 mg/kg bw of extract of isolate Sir G5). Results: The result showed that body weight rats was not significantly different at each group (P>0.05). The quantity and volume of tumor samples from isolat Sir G5 extract treatment groups was significantly lower (P<0.05) than negative control DMBA and positive control doxo. Conclusion: Overall, it can be concluded that the ethyl acetate extract of endophytic fungi isolated Sir G5 from soursop leaf (Annona muricata L.) can inhibit the growth of rat breast tumor particulary on the treatment dose DI (20 mg/Kg bw).