Wound Healing Activity of Premna latifolia Roxb.

Abstract

Aims: The study aims to understand the wound healing potentials of a 50% aqueous ethanolic extract of Premna latifolia stem using excision wound model. Study Design: The wound healing potentials were simultaneously supported by observing the bacterial functional diversity of wound swabs using Biolog Eco plates. The antioxidant activity was performed using In vitro DPPH free radical scavenging assay. Place and Duration of Study: CSIR-National Botanical Research Institute (NBRI), Lucknow, between May 2013 and November 2013. Methodology: Wound healing activity of the plant was studied using excision wound model. Animals were divided into three groups of six male rats each as control group (GI) dressed with compound free simple ointment. Test group (GII) treated with 50% aqueous ethanolic extract of P. latifolia stem (10% w/w) in ointment vehicle and standard group (GIII) group treated Nitrofurazone ointment, Himedia (0.2%w/w). The wound healing potential was further supported by the DPPH free radical scavenging and antibacterial activity of the plant. The phytochemical estimations were done using standard methods. Results: Sugar and starch content in the plant was 3.55% and 5.54% respectively. Total tannins, phenol and flavonoid content were estimated to be 0.18%, 0.54% and 2.73%. The 50% ethanolic extract of the plant showed moderate DPPH free radical scavenging activity with an IC50 of 188.02µg/ml. A 69.15% of wound closure was observed on 10th day post wounding of the rats treated with 200 mg/kg of extract. The results also indicated significant antibacterial activity of the extract. Conclusion: The 50% aqueous ethanolic extract of P. latifolia shows significant wound healing activity