Phytochemical Analysis and HPTLC Finger Printing of Nyctanthes arbor-tristis Linn. Leaf

Abstract

Herbal medicine also known as botanical medicine refers to using plants seeds, flowers, roots for medicinal purpose. Nyctanthes arbor-tristis Linn is a large shrub or small tree, found throughout in India as well as chitrakoot region.  It is commonly known as Harshingar, night jasmine, and parijat. It is used for its antibacterial, anthelmintic, anti-inflammatory, hepato-protective, immune potential, antipyretic, antioxidant and antifungal activity. The present investigation deals with preliminary phytochemical screening and High Performance Thin layer Chromatography (HPTLC) fingerprints profile of Parijat –leaf. For Phytochemical screening two various extracts (ethanolic extract and aqueous extract) of leaf were used and found the Alkaloids, Resins Saponins, Tannins, Terpinoids and Cardiac glycoside present. HPTLC finger printing were carried out on Toluene: Ethyl acetate (7:3); as a mobile phase. The plate ware developed over a distance of 9 cm in a saturated development chamber Twin through chamber 10X10 cm with SS lid and visualized under at 254nm, 366nm, under ultraviolet before and after derivatization. After spraying with 5% methanolic sulphuric acid followed by heating at 105°C for 5-10 minutes. Rf values were noted with colour before and after derivatization. Rf values are before derivatization at 366 nm 0.05, 0.27, 0.31, 0.37, 0.44, 0.51, 0.69, 0.85, 0.92, 0.96 (all red); under ultraviolet: 0.36, 0.41, 0.50, 0.55, 0.95 (light yellow). After derivatization at 366 nm 0.09 (faint white), 0.31, 0.38, 0.49 (orange), 0.58 (yellow), 0.54, 0.84 (both violet), 0.90 (light pink), 0.96(white); under ultraviolet 0.36, 0.51 (both light brown), 0.58 (light black), 0.98 (brown).